Association of leukocyte mitochondrial DNA content with glioma risk: evidence from a Chinese case-control study.
Association of leukocyte mitochondrial DNA content with glioma risk: evidence from a Chinese case-control study.
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DOI：
10.1186/1471-2407-14-680
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发表时间：
2014-09-19
期刊：
影响因子：
3.8
通讯作者：
Xing J
中科院分区：
文献类型：
--
作者：
Zhang J;Li D;Qu F;Chen Y;Li G;Jiang H;Huang X;Yang H;Xing J
越来越多的证据表明，线粒体 DNA (mtDNA) 含量的改变可能与多种恶性肿瘤的发生有关。然而，外周血淋巴细胞（PBL）中 mtDNA 含量与神经胶质瘤风险之间的关联尚未得到研究。在一项基于医院的病例对照研究中，使用实时 PCR 检测了 414 名神经胶质瘤患者和 414 名匹配对照者的 PBL 中的 mtDNA 含量。使用无条件多元逻辑回归模型评估线粒体DNA含量与神经胶质瘤风险之间的关联。我们发现神经胶质瘤患者的 mtDNA 含量中位数显着高于健康对照（0.99 vs. 0.71，P<0.001）。调整年龄、性别、吸烟状况和家族癌症史的无条件多变量逻辑回归分析表明，mtDNA 含量与神经胶质瘤风险之间存在 S 形关联。较高的线粒体 DNA 含量与神经胶质瘤的风险升高显着相关。与第一四分位数相比，线粒体DNA含量第二、第三和第四四分位数的受试者的比值比（95%置信区间）分别为0.90（0.52-1.53）、3.38（2.15-5.31）和5.81（3.74-9.03）（非线性P = 0.009）。分层分析表明，线粒体 DNA 含量与神经胶质瘤风险之间的关联不受主要宿主特征的调节。我们的研究结果首次证明，PBL 中较高的 mtDNA 含量与神经胶质瘤的风险升高相关，这值得在更大的人群中进行进一步研究。
Increasing evidence suggests that alterations in mitochondrial DNA (mtDNA) content may be implicated in the tumorigenesis of several malignancies. However, the association between mtDNA content in peripheral blood lymphocytes (PBLs) and glioma risk has not been investigated. Real-time PCR was used to examine the mtDNA content in PBLs of 414 glioma patients and 414 matched controls in a hospital-based case–control study. The association between mtDNA content and glioma risk was evaluated using an unconditional multivariate logistic regression model. We found that glioma patients exhibited a significantly higher median mtDNA content than healthy controls (0.99 vs. 0.71, P < 0.001). Unconditional multivariate logistic regression analysis adjusting for age, gender, smoking status, and family cancer history showed that there was an S-shaped association between mtDNA content and glioma risk. Higher mtDNA content was significantly associated with an elevated risk of glioma. Compared with the first quartile, the odds ratio (95% confidence interval) for subjects in the second, third, and fourth quartiles of mtDNA content were 0.90 (0.52-1.53), 3.38 (2.15-5.31), and 5.81 (3.74-9.03), respectively (P for nonlinearity = 0.009). Stratified analysis showed that the association between mtDNA content and glioma risk was not modulated by major host characteristics. Our findings demonstrate for the first time that a higher mtDNA content in PBLs is associated with an elevated risk of glioma, which warrants further investigation in larger populations.
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DOI：
10.1158/1940-6207.capr-11-0002
发表时间：
2011-11
期刊：
Cancer prevention research (Philadelphia, Pa.)
影响因子：
--
作者：
Lynch SM;Weinstein SJ;Virtamo J;Lan Q;Liu CS;Cheng WL;Rothman N;Albanes D;Stolzenberg-Solomon RZ
通讯作者：
Stolzenberg-Solomon RZ
影响因子：
9.7
作者：
Liang, BC;Hays, L
通讯作者：
Hays, L
影响因子：
6.2
作者：
Qu, Falin;Liu, Xiaonan;Xing, Jinliang
通讯作者：
Xing, Jinliang
影响因子：
4.7
作者：
Hosgood, H. Dean, III;Liu, Chin-San;Lan, Qing
通讯作者：
Lan, Qing
影响因子：
3.8
作者：
Masayesva, BG;Mambo, E;Califano, J
通讯作者：
Califano, J