Extracellular traps are associated with human and mouse neutrophil and macrophage mediated killing of larval Strongyloides stercoralis.
Extracellular traps are associated with human and mouse neutrophil and macrophage mediated killing of larval Strongyloides stercoralis.
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DOI：
10.1016/j.micinf.2014.02.012
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发表时间：
2014-06
影响因子：
5.8
通讯作者：
Abraham D
中科院分区：
文献类型：
--
作者：
Bonne-Année S;Kerepesi LA;Hess JA;Wesolowski J;Paumet F;Lok JB;Nolan TJ;Abraham D
中性粒细胞是多方面的细胞，通常是免疫系统的第一道防线。人类和小鼠细胞释放细胞外DNA陷阱（ETs），以应对几种病原体和疾病。中性粒细胞胞外陷阱（NET）的形成对捕获和杀死细胞外病原体至关重要。除了中性粒细胞，巨噬细胞和嗜酸性粒细胞也释放et。我们假设et作为一种诱捕大而高运动的粪圆线虫的机制，从而为免疫反应提供了一个静态靶点。我们证明了粪球菌幼虫触发人类中性粒细胞和巨噬细胞释放et。对蚊帐的分析表明，蚊帐能捕获但不能杀死幼虫。在体外实验中，NETs的诱导对人而非小鼠的中性粒细胞和巨噬细胞杀灭幼虫至关重要。在小鼠中，粪虫幼虫感染后诱导细胞外陷阱，并存在于体内被杀死的蠕虫的微环境中。这些发现表明，NETs诱捕寄生虫，促进免疫系统细胞杀死幼虫。
Neutrophils are multifaceted cells that are often the immune system’s first line of defense. Human and murine cells release extracellular DNA traps (ETs) in response to several pathogens and diseases. Neutrophil extracellular trap (NET) formation is crucial to trapping and killing extracellular pathogens. Aside from neutrophils, macrophages and eosinophils also release ETs. We hypothesized that ETs serve as a mechanism of ensnaring the large and highly motile helminth parasite Strongyloides stercoralis thereby providing a static target for the immune response. We demonstrated that S. stercoralis larvae trigger the release of ETs by human neutrophils and macrophages. Analysis of NETs revealed that NETs trapped but did not kill larvae. Induction of NETs was essential for larval killing by human but not murine neutrophils and macrophages in vitro. In mice, extracellular traps were induced following infection with S. stercoralis larvae and were present in the microenvironment of worms being killed in vivo. These findings demonstrate that NETs ensnare the parasite facilitating larval killing by cells of the immune system.
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影响因子：
5.5
作者：
Parker, Heather;Albrett, Amelia M.;Winterbourn, Christine C.
通讯作者：
Winterbourn, Christine C.
DOI：
10.1073/pnas.0900226106
发表时间：
2009-04-21
影响因子：
11.1
作者：
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影响因子：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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