Impact of Activation of the Neotrehalosadiamine/Kanosamine Biosynthetic Pathway on the Metabolism of Bacillus subtilis
Impact of Activation of the Neotrehalosadiamine/Kanosamine Biosynthetic Pathway on the Metabolism of Bacillus subtilis
复制标题
新海藻糖二胺/卡诺胺生物合成途径的激活对枯草芽孢杆菌代谢的影响
DOI：
10.1128/jb.00603-20
复制
发表时间：
2021
影响因子：
3.2
通讯作者：
Takashi Inaoka
中科院分区：
文献类型：
--
作者：
Natsumi Saito;Huong Minh Nguyen;Takashi Inaoka
戊糖磷酸（PP）途径是细胞NADPH的主要来源之一。缺乏葡萄糖-6-磷酸脱氢酶（催化PP途径第一步的酶）的枯草芽孢杆菌突变体显示出接种剂量依赖性生长。这种生长缺陷被glcPdisruption抑制，glcPdisruption导致自诱导物neotrehalosadiamine（NTD）/kanosamine生物合成途径上调。代谢组学分析表明，NTD/kanosamine生物合成的刺激引起三羧酸（TCA）循环中间体和NADPH的显着积累。由于主要的苹果酸酶YtsJ通过苹果酸-丙酮酸转化伴随产生NADPH，因此新NTD/卡诺胺生物合成可通过苹果酸的积累导致细胞内NADPH库的增加。事实上，azwfmutant在苹果酸补充培养基中生长。glcPin突变体的人工诱导引起细胞内NADPH库的减少。此外，NTD/kanosamine生物合成操纵子tdABC的表达水平与细胞内NADPH库之间的相关性得到证实。我们的研究结果表明，NTD/kanosamine有可能通过自诱导机制调节碳能量代谢。重要的是，自诱导物使细菌能够感知细胞密度并协调集体行为。NTD/卡诺胺是由B产生的自诱导物。枯草芽孢杆菌和几个近亲，虽然它的生理功能仍然未知。本研究最重要的发现是新NTD/kanosamine生物合成在调节B中心碳代谢中的意义。枯草杆菌。我们发现，NTD/kanosamine生物合成引起的增加，通过积累的TCA循环中间体的NADPH池。这些结果表明NTD/kanosamine在碳能量代谢中可能发挥作用。由于芽孢杆菌广泛用于工业生产各种有用的酶和化合物，NTD/kanosamine生物合成途径可能被用来控制这些工业菌株的代谢途径。
The pentose phosphate (PP) pathway is one of the major sources of cellular NADPH. A Bacillus subtiliszwfmutant that lacks glucose-6-phosphate dehydrogenase (the enzyme that catalyzes the first step of the PP pathway) showed inoculum-dose-dependent growth. This growth defect was suppressed byglcPdisruption, which causes the upregulation of the autoinducer neotrehalosadiamine (NTD)/kanosamine biosynthetic pathway. A metabolome analysis showed that the stimulation of NTD/kanosamine biosynthesis caused significant accumulation of tricarboxylic acid (TCA) cycle intermediates and NADPH. Because the major malic enzyme YtsJ concomitantly generates NADPH through malate-to-pyruvate conversion,de novoNTD/kanosamine biosynthesis can result in an increase in the intracellular NADPH pool via the accumulation of malate. In fact, azwfmutant grew in malate-supplemented medium. Artificial induction ofglcPin thezwfmutant caused a reduction in the intracellular NADPH pool. Moreover, the correlation between the expression level of the NTD/kanosamine biosynthesis operonntdABCand the intracellular NADPH pool was confirmed. Our results suggest that NTD/kanosamine has the potential to modulate carbon energy metabolism through an autoinduction mechanism.IMPORTANCEAutoinducers enable bacteria to sense cell density and to coordinate collective behavior. NTD/kanosamine is an autoinducer produced by B. subtilis and several close relatives, although its physiological function remains unknown. The most important finding of this study was the significance ofde novoNTD/kanosamine biosynthesis in the modulation of the central carbon metabolism in B. subtilis. We showed that NTD/kanosamine biosynthesis caused an increase in the NADPH pool via the accumulation of TCA cycle intermediates. These results suggest a possible role for NTD/kanosamine in carbon energy metabolism. AsBacillusspecies are widely used for the industrial production of various useful enzymes and compounds, the NTD/kanosamine biosynthetic pathway might be utilized to control metabolic pathways in these industrial strains.
登录
查看更多内容
影响因子：
3.2
作者：
Inaoka, Takashi;Ochi, Kozo
通讯作者：
Ochi, Kozo
碳水化合物的吸收和代谢
影响因子：
3.2
作者：
J. Deutscher;A. Galinier;I. Martin
通讯作者：
I. Martin
DOI：
--
发表时间：
2009
期刊：
mdtRP (yusOP) of Bacillus subtilis. 191
影响因子：
--
作者：
Kim;J. Y. T. Inaoka;K. Hirooka;H. Matsuoka;M. Murata;R. Ohki;Y. Adachi;Y. Fujita;K. Ochi.
通讯作者：
K. Ochi.
从产生布替罗辛的生物体中分离 3,3-新海藻糖二胺 (BMY-28251)。
DOI：
10.7164/antibiotics.39.1346
发表时间：
1986
期刊：
The Journal of antibiotics
影响因子：
--
作者：
K. Numata;F. Satoh;M. Hatori;T. Miyaki;H. Kawaguchi
通讯作者：
H. Kawaguchi
DOI：
10.1099/00221287-146-2-263
发表时间：
2000-02-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
Asai, K;Baik, SH;Ogasawara, N
通讯作者：
Ogasawara, N