The C. elegans Homolog of RBBP6 (RBPL-1) regulates fertility through controlling cell proliferation in the germline and nutrient synthesis in the intestine.
The C. elegans Homolog of RBBP6 (RBPL-1) regulates fertility through controlling cell proliferation in the germline and nutrient synthesis in the intestine.
复制标题
RBBP6 (RBPL-1) 的线虫同源物通过控制种系细胞增殖和肠道营养合成来调节生育力
DOI：
10.1371/journal.pone.0058736
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Miao L
中科院分区：
文献类型：
--
作者：
Huang P;Ma X;Zhao Y;Miao L
RBBP 6（视网膜母细胞瘤结合蛋白6，在人类中也称为PACT或P2P-R）是一种多结构域蛋白，在多个过程中发挥作用，如有丝分裂，细胞分化和细胞凋亡。RBBP 6在进化上是保守的，并且存在于单细胞生物到哺乳动物中。RBBP 6的研究主要集中在其RB-和p53-结合结构域，这些结构域仅在哺乳动物中发现。在这里，我们研究了C。elegans同源RBBP 6，以探索其其他结构域的功能作用。我们发现RBBP 6的同源物RBPL-1在C. elegans，是蠕虫发育不可或缺的。rbpl-1的RNAi沉默导致胚胎死亡，以及卵母细胞产生和肠发育的缺陷。rbpl-1 RNAi蠕虫显示生殖细胞增殖缺陷，表明RBPL-1调节有丝分裂。此外，rbpl-1的RNAi沉默抑制了蠕虫肠道中的营养合成。RBPL-1作为一种核仁蛋白，在多种组织中表达，是生殖细胞和索马体发育所必需的。使用微阵列分析，我们确定了约700个基因，其表达水平在rbpl-1蠕虫中至少改变了10倍。我们建议，RBPL-1，像它的酵母同系物，可以调节基因表达的mRNA切割和多聚腺苷酸化因子。综上所述，本研究的结果表明RBPL-1在C.在线虫生殖系和索马发育中，表明RBBP 6的功能在不同的真核生物中是保守的。
RBBP6 (retinoblastoma binding protein 6, also known as PACT or P2P-R in humans) is a multi-domain protein that functions in multiple processes, such as mitosis, cell differentiation, and cell apoptosis. RBBP6 is evolutionarily conserved and is present in unicellular organisms to mammals. Studies of RBBP6 have mostly focused on its RB- and p53-binding domains, which are found exclusively in mammals. Here, we investigated the C. elegans homolog of RBBP6 to explore the functional roles of its other domains. We found that RBPL-1, the homolog of RBBP6 in C. elegans, is indispensable for worm development. RNAi silencing of rbpl-1 led to embryonic lethality, as well as defects in oocyte production and intestine development. rbpl-1 RNAi worms showed defects in germ cell proliferation, suggesting that RBPL-1 regulates mitosis. Moreover, RNAi silencing of rbpl-1 inhibited nutrient synthesis in the worm intestine. RBPL-1, as a nucleolus protein, was found to be expressed in diverse tissues and necessary for both germline and soma development. Using microarray analysis, we identified ≈700 genes whose expression levels were changed at least 10-fold in rbpl-1 worms. We propose that RBPL-1, like its yeast homolog, may regulate gene expression as an mRNA cleavage and polyadenylation factor. Taken together, the findings from this study reveal that RBPL-1 plays a pivotal role in C. elegans germline and soma development, suggesting that the functions of RBBP6 are conserved in diverse eukaryotic species.
登录
查看更多内容
影响因子：
3.7
作者：
Brooks KK;Liang B;Watts JL
通讯作者：
Watts JL
影响因子：
--
作者：
Pugh, DJR;Ab, E;Rees, DJG
通讯作者：
Rees, DJG
影响因子：
3.7
作者：
Kumsta C;Hansen M
通讯作者：
Hansen M
影响因子：
64.5
作者：
Durieux J;Wolff S;Dillin A
通讯作者：
Dillin A
影响因子：
5.6
作者：
Gao, SZ;Witte, MM;Scott, RE
通讯作者：
Scott, RE