The influence of bacterial diet on fat storage in C. elegans.
The influence of bacterial diet on fat storage in C. elegans.
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DOI：
10.1371/journal.pone.0007545
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发表时间：
2009-10-21
期刊：
影响因子：
3.7
通讯作者：
Watts JL
中科院分区：
文献类型：
--
作者：
Brooks KK;Liang B;Watts JL
秀丽隐杆线虫已成为研究脂肪储存调控的重要模型。C.线虫以细菌为食，而各种E.大肠杆菌中的蛋白质通常用于研究环境。然而，目前还不知道特定的细菌饮食是否会影响脂肪储存和代谢。固定线虫的脂肪染色，以及脂质类的生化分析，揭示了C。elegans生长在四种不同的E.大肠杆菌菌株。脂肪酸组成和碳水化合物水平在E.这些研究中检测的大肠杆菌菌株，然而，这些营养差异似乎对蠕虫的脂肪储存水平没有因果影响。C.分析携带破坏神经内分泌和其它脂肪调节途径的突变的线虫菌株表明，活蠕虫的尼罗红染色强度与脂肪定量的生物化学方法没有很好的相关性。几种神经内分泌途径突变体和进食缺陷突变体显示出比野生型更高或更低的脂肪储存水平，然而，当在不同的细菌菌株上生长时，这些突变体仍然显示出脂肪储存的差异。在所有测试的突变体中，只有缺乏功能性肠肽转运蛋白的pept-1突变体没有显示出差异性脂肪储存。此外，三酰甘油储存的脂肪酸分析揭示了总脂肪储存和15-甲基棕榈酸水平之间的负相关性，来自亮氨酸催化剂。这些研究表明，在肠道中感知到的营养线索独立于神经内分泌线索调节脂肪储存水平。肽转运的参与和源自氨基酸的脂肪酸产物的积累表明，特定的肽或氨基酸可能提供调节脂肪代谢和脂肪储存水平的营养信号。
The nematode Caenorhabditis elegans has emerged as an important model for studies of the regulation of fat storage. C. elegans feed on bacteria, and various strains of E. coli are commonly used in research settings. However, it is not known whether particular bacterial diets affect fat storage and metabolism. Fat staining of fixed nematodes, as well as biochemical analysis of lipid classes, revealed considerable differences in fat stores in C. elegans growing on four different E. coli strains. Fatty acid composition and carbohydrate levels differ in the E. coli strains examined in these studies, however these nutrient differences did not appear to have a causative effect on fat storage levels in worms. Analysis of C. elegans strains carrying mutations disrupting neuroendocrine and other fat-regulatory pathways demonstrated that the intensity of Nile Red staining of live worms does not correlate well with biochemical methods of fat quantification. Several neuroendocrine pathway mutants and eating defective mutants show higher or lower fat storage levels than wild type, however, these mutants still show differences in fat stores when grown on different bacterial strains. Of all the mutants tested, only pept-1 mutants, which lack a functional intestinal peptide transporter, fail to show differential fat stores. Furthermore, fatty acid analysis of triacylglycerol stores reveals an inverse correlation between total fat stores and the levels of 15-methylpalmitic acid, derived from leucine catabolism. These studies demonstrate that nutritional cues perceived in the intestine regulate fat storage levels independently of neuroendocrine cues. The involvement of peptide transport and the accumulation of a fatty acid product derived from an amino acid suggest that specific peptides or amino acids may provide nutritional signals regulating fat metabolism and fat storage levels.
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