Leukocyte Depletion and Size-Based Enrichment of Circulating Tumor Cells Using a Pressure-Sensing Microfiltration Device.
Leukocyte Depletion and Size-Based Enrichment of Circulating Tumor Cells Using a Pressure-Sensing Microfiltration Device.
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DOI：
10.1021/acsmeasuresciau.2c00057
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发表时间：
2023-04-19
期刊：
影响因子：
--
通讯作者：
Baba, Yoshinobu
中科院分区：
文献类型：
--
作者：
Onoshima, Daisuke;Hase, Tetsunari;Kihara, Naoto;Kuboyama, Daiki;Tanaka, Hiromasa;Ozawa, Naoya;Yukawa, Hiroshi;Sato, Mitsuo;Ishikawa, Kenji;Hasegawa, Yoshinori;Ishii, Makoto;Hori, Masaru;Baba, Yoshinobu
考虑到分离循环肿瘤细胞(CTCs)的挑战与细胞应力和纯度有关，我们报告了血液微滤装置的应用，作为靶向CTCs的非侵入性液体活检的最佳方法。我们实验分析了压力传感微过滤器的过滤行为，以分离全血中的肿瘤细胞和白细胞。该方法平均可恢复96%的尖峰肿瘤细胞和99%的总白细胞。此外，我们使用血液微滤装置对CTCs进行了基因组图谱分析。该方法还应用于临床；使用少量微滤CTC进行DNA扩增，并成功检测到表皮生长因子受体突变，以表征分子靶向药物对肺癌的疗效。总体而言，提出的方法可以为评估从全血中浓缩CTCs的有效过滤压力提供一种工具。
Considering the challenges in isolating circulating tumor cells (CTCs) pertaining to cellular stress and purity, we report the application of a blood microfiltration device as an optimal approach for noninvasive liquid biopsy to target CTCs. We experimentally analyzed the filtration behavior of the microfilter using pressure sensing to separate tumor cells from leukocytes in whole blood. This approach achieved an average recovery of >96% of the spiked tumor cells and depletion of >99% of total leukocytes. Furthermore, we carried out genomic profiling of the CTCs using the blood microfiltration device. The method was also applied in a clinical setting; DNA amplification was performed using a small number of microfiltered CTCs and epidermal growth factor receptor mutations were successfully detected to characterize the efficacy of molecularly targeted drugs against lung cancer. Overall, the proposed method can provide a tool for evaluating efficient filtration pressure to concentrate CTCs from whole blood.
影响因子：
4.6
作者：
Cho W;Pradhan R;Chen HY;Weng YH;Chu HY;Tseng FG;Lin CP;Jiang JK
通讯作者：
Jiang JK