Boysenberry polyphenol inhibits endothelial dysfunction and improves vascular health.
Boysenberry polyphenol inhibits endothelial dysfunction and improves vascular health.
复制标题
DOI：
10.1371/journal.pone.0202051
复制
发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Minamino T
中科院分区：
文献类型：
--
作者：
Furuuchi R;Shimizu I;Yoshida Y;Hayashi Y;Ikegami R;Suda M;Katsuumi G;Wakasugi T;Nakao M;Minamino T
内皮细胞在维持血管稳态中具有重要作用。动脉粥样硬化相关疾病（包括肥胖症、糖尿病和高血压）或衰老本身诱导内皮功能障碍，其易于发生动脉粥样硬化。据报道，多酚类物质可抑制与年龄相关的内皮细胞疾病，但其在血管功能中的作用尚未确定。我们研究了博伊森莓多酚对饮食性肥胖小鼠模型在代谢应激下血管健康的影响。我们发现，波森莓多酚的管理抑制生产的活性氧（ROS）和增加生产的一氧化氮（NO）在主动脉。据报道，p53诱导细胞衰老，并在年龄相关疾病中起关键作用，包括心力衰竭和糖尿病。波森莓多酚的管理显着降低了主动脉内皮细胞p53水平，改善了髂动脉内皮细胞功能障碍的代谢应激。博伊森莓多酚还降低了培养的人脐静脉内皮细胞（HUVEC）中的ROS和p53水平，同时增加了NO的产生。已知解偶联内皮型一氧化氮合酶（eNOS单体）促进ROS产生。我们发现，波森莓多酚降低eNOS单体水平在体内和体外，沿着eNOS二聚化的增加。为了研究波森莓多酚介导这些有利的生物效应的组分，我们提取了花青素级分。我们发现，花青素有助于抑制ROS和p53，与增加NO的生产和eNOS二聚化。在一项离体研究中，花青素促进了饮食性肥胖小鼠髂动脉的舒张。这些研究结果表明，波森莓多酚和花青素，这种多酚的主要成分，抑制内皮功能障碍，并有助于维持血管稳态。
Endothelial cells have an important role in maintaining vascular homeostasis. Age-related disorders (including obesity, diabetes, and hypertension) or aging per se induce endothelial dysfunction that predisposes to the development of atherosclerosis. Polyphenols have been reported to suppress age-related endothelial cell disorders, but their role in vascular function is yet to be determined. We investigated the influence of boysenberry polyphenol on vascular health under metabolic stress in a murine model of dietary obesity. We found that administration of boysenberry polyphenol suppressed production of reactive oxygen species (ROS) and increased production of nitric oxide (NO) in the aorta. It has been reported that p53 induces cellular senescence and has a crucial role in age-related disorders, including heart failure and diabetes. Administration of boysenberry polyphenol significantly reduced the endothelial p53 level in the aorta and ameliorated endothelial cell dysfunction in iliac arteries under metabolic stress. Boysenberry polyphenol also reduced ROS and p53 levels in cultured human umbilical vein endothelial cells (HUVECs), while increasing NO production. Uncoupled endothelial nitric oxide synthase (eNOS monomer) is known to promote ROS production. We found that boysenberry polyphenol reduced eNOS monomer levels both in vivo and in vitro, along with an increase of eNOS dimerization. To investigate the components of boysenberry polyphenol mediating these favorable biological effects, we extracted the anthocyanin fractions. We found that anthocyanins contributed to suppression of ROS and p53, in association with increased NO production and eNOS dimerization. In an ex vivo study, anthocyanins promoted relaxation of iliac arteries from mice with dietary obesity. These findings indicate that boysenberry polyphenol and anthocyanins, a major component of this polyphenol, inhibit endothelial dysfunction and contribute to maintenance of vascular homeostasis.
登录
查看更多内容
影响因子：
5.9
作者：
Fairlie-Jones L;Davison K;Fromentin E;Hill AM
通讯作者：
Hill AM
多酚：对健康和衰老中心血管系统的好处。
影响因子：
5.9
作者：
Khurana S;Venkataraman K;Hollingsworth A;Piche M;Tai TC
通讯作者：
Tai TC
影响因子：
3.7
作者：
HAYFLICK, L;MOORHEAD, PS
通讯作者：
MOORHEAD, PS
影响因子：
15.9
作者：
Landmesser, U;Dikalov, S;Harrison, DG
通讯作者：
Harrison, DG
影响因子：
20.1
作者：
Brodsky, SV;Gealekman, O;Goligorsky, MS
通讯作者：
Goligorsky, MS