Circadian clock components in Arabidopsis III. LHY/CCA1/GI in regulating the floral integrator genes LFY/SOC1/FT to control flowering time and shoot architecture
Circadian clock components in Arabidopsis III. LHY/CCA1/GI in regulating the floral integrator genes LFY/SOC1/FT to control flowering time and shoot architecture
复制标题
拟南芥 III 中的昼夜节律时钟成分。
DOI：
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Tsuyoshi TMizoguchi
中科院分区：
文献类型：
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作者：
S. Fujiwara;M. Nakagawa;H. Kamada;Tsuyoshi TMizoguchi
LHY和CCA 1是拟南芥中的时钟元件，TFL 1编码一个茎同一性基因。当与tfl 1结合时，lhy cca 1导致花整合基因LFY的早熟和异位表达。我们已经表明，lhy cca 1和GI-ox导致提前开花，并增加两个花整合基因，FT和SOC 1的表达。FT和SOC 1是SD条件下GI-ox提早开花所必需的。在这里，我们表明，tfl 1显着减少代时间的GI-OX以及LHY CCA 1植物。tfl 1以类似于lhy cca 1的方式增强GI-ox中分生组织身份基因AP 1的早熟表达。然而，tfl 1不影响lhy cca 1和GI-ox中FT和SOC 1的mRNA水平，表明lhy cca 1 tfl 1（和GI-ox tfl 1）中的相加表型可归因于FT/SOC 1和LFY三个基因的同时上调。在SD条件下，lhy cca 1和GI-ox增强了TFL 1的顶生花表型，表明FT和TFL 1之间具有拮抗作用的适当平衡对于拟南芥的光周期结构控制非常重要。我们的研究结果表明，GI介导的生物钟和三个花整合基因，FT，SOC 1和LFY，控制光周期开花。
LHY and CCA1 are clock components and TFL1 encodes a shoot-identity gene in Arabidopsis. When combined with tfl1, lhy cca1 results in precocious and ectopic expression of a floral integrator gene, LFY. We have shown that lhy cca1 and GI-ox cause early flowering and increase the expression of two floral integrator genes, FT and SOC1. FT and SOC1 are required for the early flowering of GI-ox under SD. Here we demonstrate that tfl1 dramatically reduced the generation time of GI-ox as well as lhy cca1 plants. tfl1 enhanced the precocious expression of the meristem-identity gene AP1 in GI-ox in a similar way to that in lhy cca1. However, tfl1 did not affect the mRNA levels of FT and SOC1 in lhy cca1 and GI-ox, suggesting that the additive phenotypes in lhy cca1 tfl1 (and GI-ox tfl1) are attributable to the concurrent up- regulation of three genes, FT/SOC1 and LFY. The terminal flower phenotype of tfl1 was enhanced by lhy cca1 and GI-ox under SD, suggesting that a proper balance between FT and TFL1 with antagonistic roles is important for the photoperiodic control of architecture in Arabidopsis. Our results indicate that GI mediates between the circadian clock and three floral integrator genes, FT, SOC1 and LFY, to control the photoperiodic flowering.
影响因子：
4.9
作者：
Nakagawa, M;Komeda, Y
通讯作者：
Komeda, Y