Combination of conventional immunohistochemistry and qRT-PCR to detect ALK rearrangement.
Combination of conventional immunohistochemistry and qRT-PCR to detect ALK rearrangement.
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DOI：
10.1186/1746-1596-9-3
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发表时间：
2014-01-14
影响因子：
2.6
通讯作者：
Lin D
中科院分区：
文献类型：
--
作者：
Shan L;Lian F;Guo L;Yang X;Ying J;Lin D
与FISH和qRT-PCR分析相比，免疫组织化学（IHC）是大多数病理学实践中ALK重排检测的首选筛选试验。VENTANA ALK（D5 F3）IHC检测试剂盒具有100%的灵敏度和98%的特异性，已在欧盟和一些亚洲国家获批用于ALK重排检测。然而，自动化Ventana IHC平台在大多数病理学实验室中不可用。在这项研究中，我们评估了D5 F3抗体的常规IHC在常规病理实践中的适用性，并提出了确保不遗漏ALK+患者的检测方法和程序。对297例肺腺癌进行了FISH和IHC分析。采用VENTANA IHC和qRT-PCR检测评价FISH和IHC不一致病例中的ALK融合状态。统计分析ALK+与临床病理特征的相关性。IHC检测ALK+的敏感性为100%，特异性为81.8%。8例ALK表达病例为ALK-，其中5例通过qRT-PCR分析检测到ALK融合。使用VENTANA IHC检测，这5例病例中有3例显示ALK表达。在该中国肺腺癌患者队列中，ALK+与年龄较小和淋巴结转移相关。低成本和100%灵敏度的优势使传统IHC成为筛查ALK+患者的强大诊断工具，特别是在没有VENTANA IHC平台的病理学实验室中。对于ALK弱表达的病例，需要qRT-PCR作为ALK融合检测的诊断检测。本文的虚拟幻灯片可以在这里找到：http://www.diagnosticpathology.diagnomx.eu/vs/2269448351088278。
Compared with FISH and qRT-PCR analyses, immunohistochemistry (IHC) is the preferred screening test in most pathology practices for ALK-rearrangement detection. With 100% sensitivity and 98% specificity, the VENTANA ALK (D5F3) IHC assay has been approved in the EU and some Asian countries for ALK-rearrangement detection. However, an automated Ventana IHC platform is not available in most pathology labs. In this study, we evaluated the applicability of conventional IHC with D5F3 antibody in routine pathological practice and proposed detection methods and procedures that ensure that patients with ALK+ are not missed. FISH and IHC analyses were performed on 297 lung adenocarcinoma cases. VENTANA IHC and qRT-PCR assay were applied to evaluate ALK-fusion status in the discordant cases of FISH and IHC. The association of ALK+ with clinicopathological characteristics was statistically analyzed. IHC had 100% sensitivity and 81.8% specificity for detecting ALK+. Eight ALK-expressed cases were ALK-, five of which had ALK fusion detected by qRT-PCR analysis. Three of these five cases showed ALK expression using VENTANA IHC assay. ALK+ was associated with younger age and lymph node metastasis in this Chinese lung adenocarcinoma patient cohort. The advantages of low cost and 100% sensitivity allow conventional IHC to serve as a robust diagnostic tool for screening patients with ALK+, especially in pathology labs without a VENTANA IHC platform. For cases in which ALK is weakly expressed, qRT-PCR is necessary as a diagnostic test for ALK-fusion detection. The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/2269448351088278.
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中国ALK重排肺癌：临床病理学、IHC、FISH和RT-PCR的综合评估
影响因子：
3.7
作者：
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通讯作者：
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影响因子：
3.4
作者：
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Tsujimoto, Masahiko