Investigation of Differences in Fertility among Progenies from Self-Pollinated Chrysanthemum.
Investigation of Differences in Fertility among Progenies from Self-Pollinated Chrysanthemum.
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自花授粉菊花后代受性差异的调查
DOI：
10.3390/ijms19030832
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发表时间：
2018-03-13
影响因子：
5.6
通讯作者：
Teng N
中科院分区：
文献类型：
--
作者：
Wang F;Zhong X;Wang H;Song A;Chen F;Fang W;Jiang J;Teng N
大多数菊花品种都是自交不亲和的，因此很难培育出在菊花育种和理论研究中具有重要意义的纯种。在我们之前的研究中，我们获得了一个自交的菊花品种，其自花授粉的结实率为56.50%。有趣的是，其10个子代的种子集在0% ~ 37.23%之间。研究造成结实率差异的因素将有助于提高对菊花自交不亲和的认识，并为创建纯种提供有价值的信息。采用离体培养法、石蜡切片技术、扫描电镜和透射电镜对花粉形态、花粉发芽率、雌蕊接受度和胚胎发育进行了研究。此外，利用RNA测序和生物信息学分析了成熟柱头和花药的转录组学特征。结果表明，“Q10-33-1①”、“Q10-33-1③”、“Q10-33-1④”和“Q10-33-1⑩”自花授粉种子集分别为37.23%、26.77%、7.97%和0%。4个子代的育性差异主要是由于花粉发芽率和雌蕊接受度的差异。在“Q10-33-1”中设置的种子失败可能是由于自不相容。在转录组学文件中，鉴定出22个潜在柱头S基因和8个潜在花粉S基因。
Most chrysanthemum cultivars are self-incompatible, so it is very difficult to create pure lines that are important in chrysanthemum breeding and theoretical studies. In our previous study, we obtained a self-compatible chrysanthemum cultivar and its self-pollinated seed set was 56.50%. It was interesting that the seed set of its ten progenies ranged from 0% to 37.23%. Examination of the factors causing the differences in the seed set will lead to an improved understanding of chrysanthemum self-incompatibility, and provide valuable information for creating pure lines. Pollen morphology, pollen germination percentage, pistil receptivity and embryo development were investigated using the in vitro culture method, the paraffin section technique, scanning electron microscopy and transmission electron microscopy. Moreover, RNA sequencing and bioinformatics were applied to analyzing the transcriptomic profiles of mature stigmas and anthers. It was found that the self-pollinated seed set of “Q10-33-1①”,”Q10-33-1③”,”Q10-33-1④” and “Q10-33-1⑩” were 37.23%, 26.77%, 7.97% and 0%, respectively. The differences in fertility among four progenies were mainly attributable to differences in pollen germination percentage and pistil receptivity. Failure of the seed set in “Q10-33-1⑩” was possibly due to self-incompatibility. In the transcriptomic files, 22 potential stigma S genes and 8 potential pollen S genes were found out.
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影响因子：
3.3
作者：
Chong X;Zhang F;Wu Y;Yang X;Zhao N;Wang H;Guan Z;Fang W;Chen F
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通讯作者：
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作者：
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通讯作者：
Holtsford, Timothy P.