Molecular epidemiology and genetic diversity of Orientia tsutsugamushi from patients with scrub typhus in 3 regions of India.
Molecular epidemiology and genetic diversity of Orientia tsutsugamushi from patients with scrub typhus in 3 regions of India.
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DOI：
10.3201/eid2101.140580
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发表时间：
2015-01
影响因子：
11.8
通讯作者：
Abraham OC
中科院分区：
文献类型：
--
作者：
Varghese GM;Janardhanan J;Mahajan SK;Tariang D;Trowbridge P;Prakash JA;David T;Sathendra S;Abraham OC
澄清局部抗原多样性对于开发区域特异性疫苗和诊断方法至关重要。恙虫病是一种在亚太地区广泛传播的急性发热性疾病，由恙虫病东方体细菌引起，该细菌表现出高水平的抗原变异。我们进行了一项调查，以确定印度 3 个丛林斑疹伤寒流行地理区域（印度南部、印度北部和印度东北部）中恙虫病流行的基因型。 2010 年 9 月至 2012 年 8 月从恙虫病患者采集的焦痂样本进行了 56 kDa 类型特异性 PCR 和测序，以鉴定其基因型。 Kato 样菌株占主导地位（61.5%），特别是在南部和东北部，其次是 Karp 样菌株（27.7%）以及 Gilliam 和 Ikeda 菌株（各 2.3%）。东北地区也发现了Neimeng-65基因型菌株。阐明印度恙虫病的基因型多样性可以增强对流行毒株区域多样性的了解，并为未来区域特异性诊断研究和疫苗开发提供潜在资源。
Clarifying local antigenic diversity is critical for development of region-specific vaccines and diagnostics. Scrub typhus, an acute febrile illness that is widespread in the Asia-Pacific region, is caused by the bacterium Orientia tsutsugamushi, which displays high levels of antigenic variation. We conducted an investigation to identify the circulating genotypes of O. tsutsugamushi in 3 scrub typhus–endemic geographic regions of India: South India, Northern India, and Northeast India. Eschar samples collected during September 2010–August 2012 from patients with scrub typhus were subjected to 56-kDa type-specific PCR and sequencing to identify their genotypes. Kato-like strains predominated (61.5%), especially in the South and Northeast, followed by Karp-like strains (27.7%) and Gilliam and Ikeda strains (2.3% each). Neimeng-65 genotype strains were also observed in the Northeast. Clarifying the genotypic diversity of O. tsutsugamushi in India enhances knowledge of the regional diversity among circulating strains and provides potential resources for future region-specific diagnostic studies and vaccine development.
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影响因子：
11.8
作者：
Mahajan SK;Rolain JM;Kashyap R;Bakshi D;Sharma V;Prasher BS;Pal LS;Raoult D
通讯作者：
Raoult D
影响因子：
2.7
作者：
Bakshi, Diprabhanu;Singhal, Pradeep;Batra, Harsh Vardhan
通讯作者：
Batra, Harsh Vardhan
影响因子：
--
作者：
Blacksell, Stuart D.;Luksameetanasan, Rungnapa;Day, Nicholas P. J.
通讯作者：
Day, Nicholas P. J.
影响因子：
2.1
作者：
Enatsu, T;Urakami, HL;Tamura, A
通讯作者：
Tamura, A
影响因子：
1.7
作者：
Saifudheen, K.;Kumar, K. G. Sajeeth;Gafoor, V. Abdul
通讯作者：
Gafoor, V. Abdul