Genome-wide excision repair in Arabidopsis is coupled to transcription and reflects circadian gene expression patterns.
Genome-wide excision repair in Arabidopsis is coupled to transcription and reflects circadian gene expression patterns.
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DOI：
10.1038/s41467-018-03922-5
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发表时间：
2018-04-17
影响因子：
16.6
通讯作者：
Adebali O
中科院分区：
文献类型：
--
作者：
Oztas O;Selby CP;Sancar A;Adebali O
植物暴露于许多DNA损伤应力，包括暴露于太阳辐射的紫外线（UV）成分。它们利用核苷酸切除修复来去除DNA庞大的加合物并帮助消除紫外线诱导的DNA损伤，以保持其基因组的完整性和适应性。在这里，我们产生了全基因组的单核苷酸分辨率切除修复图谱的紫外线诱导的DNA损伤在拟南芥在不同的昼夜节律的时间点。我们的数据表明，大部分基因组的紫外线损伤的修复是由生物钟和RNA聚合酶II转录的联合作用控制的。我们的研究结果揭示了拟南芥中活性基因的转录链具有非常强的修复偏好，并且10-30%的转录偶联修复是昼夜节律时间依赖的。这种核苷酸切除修复水平的动态范围使拟南芥能够科普包括UV在内的大量DNA损伤诱导环境因素。植物利用核苷酸切除修复来维持基因组完整性，以响应由诸如UV辐射的胁迫引起的损伤。在这里，Oztas等人使用全基因组分析表明，拟南芥中的切除修复与转录密切相关，并反映了基因表达的昼夜节律模式。
Plants are exposed to numerous DNA-damaging stresses including the exposure to ultraviolet (UV) component of solar radiation. They employ nucleotide excision repair to remove DNA-bulky adducts and to help eliminate UV-induced DNA lesions, so as to maintain their genome integrity and their fitness. Here, we generated genome-wide single-nucleotide resolution excision repair maps of UV-induced DNA damage in Arabidopsis at different circadian time points. Our data show that the repair of UV lesions for a large fraction of the genome is controlled by the joint actions of the circadian clock and transcription by RNA polymerase II. Our findings reveal very strong repair preference for the transcribed strands of active genes in Arabidopsis, and 10–30% of the transcription-coupled repair is circadian time-dependent. This dynamic range in nucleotide excision repair levels throughout the day enables Arabidopsis to cope with the bulky DNA lesion-inducing environmental factors including UV. Plants use nucleotide excision repair to maintain genome integrity in response to damage caused by stresses such as UV radiation. Here Oztas et al. use genome-wide profiling to show that excision repair in Arabidopsis is strongly coupled to transcription and reflects circadian patterns of gene expression.
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影响因子：
20.5
作者：
Hsu, Polly Yingshan;Harmer, Stacey L.
通讯作者：
Harmer, Stacey L.
影响因子：
56.9
作者：
Harmer, SL;Hogenesch, LB;Kay, SA
通讯作者：
Kay, SA
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Pikaard, Craig S.;Scheid, Ortrun Mittelsten
通讯作者：
Scheid, Ortrun Mittelsten
优先修复植物中转录的DNA链。
影响因子：
5.6
作者：
Fidantsef AL;Britt AB
通讯作者：
Britt AB
影响因子：
12.3
作者：
Covington, Michael F.;Maloof, Julin N.;Straume, Marty;Kay, Steve A.;Harmer, Stacey L.
通讯作者：
Harmer, Stacey L.