Allocation of a powdery mildew resistance locus to the chromosome arm 6RL of Secale cereale L. cv. ‘Jingzhouheimai’
Allocation of a powdery mildew resistance locus to the chromosome arm 6RL of Secale cereale L. cv. ‘Jingzhouheimai’
复制标题
将白粉病抗性基因座分配至黑麦 L. cv. 的染色体臂 6RL。
DOI：
10.1007/s10681-010-0199-7
复制
发表时间：
2010-05
期刊：
影响因子：
1.9
通讯作者：
亓增军
中科院分区：
文献类型：
--
作者：
王丹;孙玲;庄丽芳;裴自友;冯祎高;亓增军
中国品种荆州黑麦对几种重要的小麦病害具有抗性，是小麦改良的有益资源。以荆州黑麦为材料，与六倍体小麦地方品种惠贤红杂交，将F1染色体数加倍，合成八倍体两倍体（2n = 8x = 56； AABBDDRR）。该两倍体与小麦亲本回交产生小麦-黑麦染色体附加系。用16个黑麦特异性DNA标记对现有的6个二体附加系（除6R外）进行筛选，鉴定出3个推测的6R标记。这些标记被用于染色体6R附加系的选择，并经基因组原位杂交（GISH）证实。通过这种方法，选择了7个单体6R和5个二体添加物，以及1个单端体6RS添加物，1个单端体和1个二端体6RL添加物。这些6R附加系又被用来开发额外的15个6R特异性标记，其中6个分配给6RS组，9个分配给6RL组。筛选白粉病抗性鉴定染色体臂6RL为抗性位点的载体。因此，鉴定为6RL臂特异性的DNA标记可用于监测抗性位点向小麦的渗入。
Chinese cultivar Jingzhouheimai of Secale cereale L. confers resistance to several important wheat diseases, making it a useful resource for wheat improvement. Octoploid amphiploid (2n = 8x = 56; AABBDDRR) was synthesized by hybridization of Jingzhouheimai with hexaploid wheat landrace Huixianhong and doubling of the F1 chromosome number. This amphiploid was backcrossed to the wheat parent to produce wheat-rye chromosome addition lines. The existing six disomic addition lines (all except 6R) were screened with 16 rye-specific DNA markers and three putative markers for 6R were identified. These markers were used in selection of chromosome 6R addition lines, confirmed by the genomic in situ hybridization (GISH). In this manner, seven monosomic 6R and five disomic addition were selected, as well as one mono-telosomic 6RS addition, one mono-telosomic and one ditelosomic 6RL additions. In turn these 6R addition lines were used to develop additional 15 6R-specific markers of which six were allocated to the arm 6RS and nine to 6RL. Screening for the powdery mildew resistance identified chromosome arm 6RL as the carrier of the resistance locus. Therefore, DNA markers identified as specific to the 6RL arm can be used to monitor the introgression of the resistance locus into wheat.
登录
查看更多内容
影响因子：
1.9
作者：
R. Villareal;E. Toro;S. Rajaram;A. Mujeeb-Kazi
通讯作者：
R. Villareal;E. Toro;S. Rajaram;A. Mujeeb-Kazi
影响因子：
3.1
作者：
B. Saal;G. Wricke
通讯作者：
B. Saal;G. Wricke
DOI：
--
发表时间：
2005
期刊：
--
影响因子：
--
作者：
Feng Chen;Senhe Qian;Yan Zhang;X. Xia;Zhonghu He
通讯作者：
Feng Chen;Senhe Qian;Yan Zhang;X. Xia;Zhonghu He
影响因子：
3.1
作者：
MCINTYRE, CL;PEREIRA, S;APPELS, R
通讯作者：
APPELS, R
影响因子：
2.6
作者：
Cuadrado, Angeles;Jouve, Nicolas
通讯作者：
Jouve, Nicolas