Validation of excitation-scan hyperspectral multi-faceted mirror array prototype system advancements to hyperspectral imaging applications
Validation of excitation-scan hyperspectral multi-faceted mirror array prototype system advancements to hyperspectral imaging applications
复制标题
验证激发扫描高光谱多面镜阵列原型系统在高光谱成像应用中的进步
DOI：
10.1117/12.2607659
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Leavesley, Silas J.
中科院分区：
文献类型：
--
作者：
Parker, Marina;Mayes, Sam A.;Browning, Craig M.;Deal, Joshua;Gunn-Mayes, Samantha;Rich, Thomas C.;Leavesley, Silas J.
高光谱成像技术（HSI）自诞生以来经历了快速的发展。虽然最初的应用是遥感，但其他用途包括农业、食品安全和医药。HSI在荧光显微镜中显示出很大的实用性，用于检测来自许多荧光分子的特征;然而，由于与光谱过滤相关的光损失，采集速度很慢。因此，我们设计了一种新型的基于发光二极管（LED）的快速激发扫描高光谱成像平台，允许用户在不影响采集速度的情况下获得荧光标记的同时测量。以前，我们报告了我们的结果的光线跟踪模拟和几何能力的设计多面镜成像系统作为一种初始方法，以联合收割机在许多波长的光。该设计利用LED和多面镜阵列将联合收割机光源组合成液体光导。使用蒙特卡罗光学射线软件（TracePro，Lambda Research Corp.）构建计算模型。最近的原型验证结果表明，当与商业发射扫描光谱共聚焦显微镜（Zeiss-LSM-980）相比，新的基于LED的激发扫描HSI原型成功地检测和分离6个荧光标记从定制的6-标记非洲绿色猴肾上皮细胞。我们报告的原型的能力，以克服在传统系统中存在的采集速度，灵敏度和特异性的限制。未来的工作将评估原型的光损失，以确定潜在的设计修改需要证明系统的可行性，作为一个有前途的解决方案，克服HSI采集速度。这项工作得到了NSF奖MRI 1725937的支持。
Hyperspectral imaging technologies (HSI) have undergone rapid development since their beginning stages. While original applications were in remote sensing, other uses include agriculture, food safety and medicine. HSI has shown great utility in fluorescence microscopy for detecting signatures from many fluorescent molecules; however, acquisitions speeds have been slow due to light losses associated with spectral filtering. Therefore, we designed a novel light emitting diode (LED)-based rapid excitation scanning hyperspectral imaging platform allowing users to obtain simultaneous measurements of fluorescent labels without compromising acquisition speeds. Previously, we reported our results of the optical ray trace simulations and the geometrical capability of designing a multifaceted mirror imaging system as an initial approach to combine light at many wavelengths. The design utilized LEDs and a multifaceted mirror array to combine light sources into a liquid light guide. The computational model was constructed using Monte Carlo optical ray software (TracePro, Lambda Research Corp.). Recent prototype validation results show that when compared to a commercial emission scanning spectral confocal microscope (Zeiss-LSM-980), the novel LED-based excitation scanning HSI prototype successfully detected and separated six fluorescent labels from a custom 6-label African green monkey kidney epithelial cells. We report on the prototype’s ability to overcome limitations of acquisition speeds, sensitivity, and specificity present in conventional systems. Future work will evaluate prototype’s light losses to determine latent design modifications needed to demonstrate the system’s feasibility as a promising solution for overcoming HSI acquisition speeds. This work was supported by NSF award MRI1725937.
登录
查看更多内容
DOI：
10.1007/978-1-60761-404-3_11
发表时间：
2010-01-01
期刊：
LIVE CELL IMAGING: METHODS AND PROTOCOLS
影响因子：
--
作者：
Wurm, Christian A.;Neumann, Daniel;Jakobs, Stefan
通讯作者：
Jakobs, Stefan
影响因子：
3.5
作者：
Favreau, Peter F.;Hernandez, Clarissa;Leavesley, Silas J.
通讯作者：
Leavesley, Silas J.
影响因子：
1.2
作者：
Deal, Joshua;Britain, Andrea;Leavesley, Silas
通讯作者：
Leavesley, Silas
影响因子：
4
作者：
Heddleston, John M;Aaron, Jesse S;Chew, Teng-Leong
通讯作者：
Chew, Teng-Leong
影响因子：
3.5
作者：
Favreau, Peter;Hernandez, Clarissa;Leavesley, Silas J.
通讯作者：
Leavesley, Silas J.