Overexpression of Grb2-associated binder 2 in human lung cancer.
Overexpression of Grb2-associated binder 2 in human lung cancer.
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DOI：
10.7150/ijbs.7.496
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发表时间：
2011-04-18
影响因子：
9.2
通讯作者：
Li JW
中科院分区：
文献类型：
--
作者：
Xu XL;Wang X;Chen ZL;Jin M;Yang W;Zhao GF;Li JW
目的：Grb2-associated binder 2 （Gab2）是gabb支架接头家族的一员，其作用是传递和放大来自受体酪氨酸激酶的信号。最近的一项研究表明，Gab2在乳腺癌和转移性黑色素瘤中过度表达，Gab2是一种致癌蛋白。然而，Gab2在肺癌中的作用在很大程度上是未知的。方法：为了探讨Gab2的表达是否可能是肺癌的一个特征，本研究采用定量实时荧光定量pcr、免疫组织化学和western blot技术分析了88例肺组织冷冻标本和122例石蜡包埋组织标本中Gab2的表达。结果：我们发现，经免疫组化检测，肿瘤组织中Gab2的阳性表达率在鳞状细胞癌中为62.5%，在腺癌中为51.35%，在其他类型肺癌中为75%，明显高于正常肺组织（12%）。实时荧光定量pcr检测各组mRNA表达和western blotting检测各组蛋白表达与免疫组化结果一致。结论：我们的数据表明，与正常肺组织相比，Gab2在恶性肺组织中过表达，提示Gab2的表达可能在肺癌的发生发展中发挥作用。
Objective: Grb2-associated binder 2 (Gab2), a member of the family of Gab scaffolding adaptors, transmits and amplifies the signals from receptor tyrosine kinases. A recent study demonstrated that Gab2 was over-expressed in breast cancers and metastatic melanomas, and Gab2 was an oncogenic protein. However, the roles of Gab2 in lung cancers are largely unknown. Method: In this study, to investigate whether Gab2 expression could be a characteristic of lung cancers, we analyzed the expression of Gab2 in 88 lung frozen tissue samples and 122 paraffin-embedded tissue specimens, using quantitative real-time-PCR, immunohistochemistry and western blot. Results: We found that the positive expression rate of Gab2 in the tumor tissues, as detected by immunohistochemistry, 62.5% in squamous cell cancers, 51.35% in adenocarcinomas, and 75% in other types of lung cancers, was significantly higher than that (12%) in normal lung tissues. The mRNA expression detected by quantitative real-time-PCR and protein expression detected by western blotting in different groups were consistent with the immunohistochemical results. Conclusion: Our data indicate that Gab2 is over-expressed in malignant lung tissues compared with that in normal lung tissues, and suggest that Gab2 expression may play a role in lung cancer development.
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影响因子：
8
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影响因子：
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作者：
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