Passive and Flexible Wireless Electronics Fabricated on Parylene/PDMS Substrate for Stimulation of Human Stem Cell-Derived Cardiomyocytes.
Passive and Flexible Wireless Electronics Fabricated on Parylene/PDMS Substrate for Stimulation of Human Stem Cell-Derived Cardiomyocytes.
复制标题
在聚对二甲苯/PDMS 基底上制造的无源柔性无线电子器件，用于刺激人类干细胞衍生的心肌细胞。
DOI：
10.1021/acssensors.2c00794
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发表时间：
2022-11-25
期刊：
影响因子：
8.9
通讯作者：
Christen, Jennifer Blain
中科院分区：
文献类型：
--
作者：
Benbuk, Ahmed Abed;Esmaeili, Hamid;Liu, Shiyi;Patino-Guerrero, Alejandra;Migrino, Raymond Q.;Chae, Junseok;Nikkhah, Mehdi;Christen, Jennifer Blain
关键词：
在本文中，我们报告了一种无线、被动、生物相容且灵活的系统的开发，用于刺激人类诱导多能干细胞衍生的心肌细胞（hiPSC-CMS）。所提出的刺激器在透明的聚对二甲苯/PDMS 基板上制造，能够对板上培养的 hiPSC-CM 进行实时激发和表征。该设备包括一个工作频率为 2.35 GHz 的整流天线，接收来自外部发射器的射频 (RF) 能量，并将其转换为直流电压以提供单相刺激。刺激器的操作主要通过金电极向培养在基质胶涂层基质上的 hiPSC-CM 传递单相电压脉冲来验证。当以 0.5、1 和 2 Hz 脉冲频率传递时，受刺激的 hiPSC-CM 根据单相脉冲跳动，而没有观察到明显的细胞死亡。无线刺激器可以在 15 mm 的距离处产生幅度为 8 V 的单相脉冲。这些结果证明了所提出的无线刺激器为工程心脏组织提供电刺激的功效。所提出的刺激器将在需要对导电细胞进行完全无线刺激的组织工程中具有广泛的应用。该设备还有可能通过提供外部刺激和调节心脏组织的收缩而用作心脏刺激器。
In this paper, we report the development of a wireless, passive, biocompatible, and flexible system for stimulation of human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMS). Fabricated on a transparent parylene/PDMS substrate, the proposed stimulator enables real-time excitation and characterization of hiPSC-CMs cultured on-board. The device comprises a rectenna operating at 2.35 GHz which receives radio frequency (RF) energy from an external transmitter and converts it into DC voltage to deliver monophasic stimulation. The operation of the stimulator was primarily verified by delivering monophasic voltage pulses through gold electrodes to hiPSC-CMs cultured on the Matrigel-coated substrates. Stimulated hiPSC-CMs beat in accordance with the monophasic pulses when delivered at 0.5, 1, and 2 Hz pulsing frequency, while no significant cell death was observed. The wireless stimulator could generate monophasic pulses with an amplitude of 8 V at a distance of 15 mm. These results demonstrated the proposed wireless stimulator’s efficacy for providing electrical stimulation to engineered cardiac tissues. The proposed stimulator will have a wide application in tissue engineering where a fully wireless stimulation of electroconductive cells is needed. The device also has potential to be employed as a cardiac stimulator by delivering external stimulation and regulating the contractions of cardiac tissue.
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