From OCR and ECAR to energy: Perspectives on the design and interpretation of bioenergetics studies.
From OCR and ECAR to energy: Perspectives on the design and interpretation of bioenergetics studies.
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DOI：
10.1016/j.jbc.2021.101140
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发表时间：
2021-10
期刊：
影响因子：
--
通讯作者：
Neufer PD
中科院分区：
文献类型：
--
作者：
Schmidt CA;Fisher-Wellman KH;Neufer PD
生物能量转换是细胞中所有生理现象的基础。支持能量转导的代谢系统由于其与包括糖尿病、癌症、罕见遗传疾病和异常细胞死亡在内的许多病理学相关而引起极大兴趣。商业上可获得的生物能量学技术（例如，细胞外流量分析、高分辨率呼吸测定法、荧光染料试剂盒等）已经使代谢参数的实际评估广泛可用。这促进了研究数量的爆炸，特别是探索耗氧速率（OCR）和通过糖酵解的底物水平磷酸化的生物学意义（即，通过胞外酸化率（ECAR））。虽然这些技术已经证明了对细胞生物学研究的巨大实用性和广泛适用性，但它们也容易受到历史假设，实验限制和其他警告的影响，导致过早和/或错误的解释。本文综述了设计和解释细胞和线粒体生物能量学实验的各种重要考虑因素，数据解释中的一些常见挑战和陷阱，以及可以解决这些突出挑战的一些潜在的“下一步”。
Biological energy transduction underlies all physiological phenomena in cells. The metabolic systems that support energy transduction have been of great interest due to their association with numerous pathologies including diabetes, cancer, rare genetic diseases, and aberrant cell death. Commercially available bioenergetics technologies (e.g., extracellular flux analysis, high-resolution respirometry, fluorescent dye kits, etc.) have made practical assessment of metabolic parameters widely accessible. This has facilitated an explosion in the number of studies exploring, in particular, the biological implications of oxygen consumption rate (OCR) and substrate level phosphorylation via glycolysis (i.e., via extracellular acidification rate (ECAR)). Though these technologies have demonstrated substantial utility and broad applicability to cell biology research, they are also susceptible to historical assumptions, experimental limitations, and other caveats that have led to premature and/or erroneous interpretations. This review enumerates various important considerations for designing and interpreting cellular and mitochondrial bioenergetics experiments, some common challenges and pitfalls in data interpretation, and some potential “next steps” to be taken that can address these highlighted challenges.
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影响因子：
5.6
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1073/pnas.76.5.2350
发表时间：
1979-01-01
影响因子：
11.1
作者：
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通讯作者：
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