Identification of Ligularia Herbs Using the Complete Chloroplast Genome as a Super-Barcode.
Identification of Ligularia Herbs Using the Complete Chloroplast Genome as a Super-Barcode.
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DOI：
10.3389/fphar.2018.00695
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发表时间：
2018
影响因子：
5.6
通讯作者：
Yao H
中科院分区：
文献类型：
--
作者：
Chen X;Zhou J;Cui Y;Wang Y;Duan B;Yao H
30余种藤本植物。(菊科)植物在中国民间医药中的应用由来已久。形态特征和共同的DNA区域都不能很好地用于植物的鉴定。由于一些植物含有吡咯里西丁生物碱，对人和动物的健康有害，并参与肝脏的代谢毒性，因此找到一种更好的方法来区分这些物种是很重要的。在这里，我们报道了通过高通量灯盏花测序技术获得的六个种的叶绿体(CP)基因组：中间连翘、连翘连翘、蒙古连翘连翘、霍氏连翘连翘、连翘连翘和连翘连翘。这些CP基因组呈典型的圆形四聚体结构，大小在151,118~151,253个碱基之间。每个CP基因组的GC含量为37.5%。每个CP基因组包含134个基因，包括87个蛋白质编码基因、37个tRNA基因、8个rRNA基因和两个假基因(ycf1和rps19)。从mVISTA来看，没有潜在的编码区或非编码区来区分这6个种，但这6个种与其他相关物种的最大似然树表明，整个CP基因组可以作为一个超级条形码来区分这6个种。这项研究为物种鉴定提供了宝贵的数据，为今后系统进化和安全医学应用的研究提供了可能。
More than 30 Ligularia Cass. (Asteraceae) species have long been used in folk medicine in China. Morphological features and common DNA regions are both not ideal to identify Ligularia species. As some Ligularia species contain pyrrolizidine alkaloids, which are hazardous to human and animal health and are involved in metabolic toxification in the liver, it is important to find a better way to distinguish these species. Here, we report complete chloroplast (CP) genomes of six Ligularia species, L. intermedia, L. jaluensis, L. mongolica, L. hodgsonii, L. veitchiana, and L. fischeri, obtained through high-throughput Illumina sequencing technology. These CP genomes showed typical circular tetramerous structure and their sizes range from 151,118 to 151,253 bp. The GC content of each CP genome is 37.5%. Every CP genome contains 134 genes, including 87 protein-coding genes, 37 tRNA genes, eight rRNA genes, and two pseudogenes (ycf1 and rps19). From the mVISTA, there were no potential coding or non-coding regions to distinguish these six Ligularia species, but the maximum likelihood tree of the six Ligularia species and other related species showed that the whole CP genome can be used as a super-barcode to identify these six Ligularia species. This study provides invaluable data for species identification, allowing for future studies on phylogenetic evolution and safe medical applications of Ligularia.
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