Soluble tumor necrosis factor related apoptosis inducing ligand level as a predictor of severity of sepsis and the risk of mortality in septic patients.
Soluble tumor necrosis factor related apoptosis inducing ligand level as a predictor of severity of sepsis and the risk of mortality in septic patients.
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可溶性肿瘤坏死因子相关凋亡诱导配体水平作为脓毒症严重程度和脓毒症患者死亡风险的预测因子
DOI：
10.1371/journal.pone.0082204
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Deng X
中科院分区：
文献类型：
--
作者：
Tian Y;Tao T;Zhu J;Zou Y;Wang J;Li J;Bo L;Deng X
背景肿瘤坏死因子相关凋亡诱导配体(TRAIL)作为肿瘤坏死因子基因超家族的成员，主要诱导肿瘤细胞的凋亡。TRAIL在炎症反应的调节中也起着重要作用，特别是在免疫麻痹过程中。本研究的目的是检测脓毒症患者的可溶性TRAIL(STRAIL)水平，试图探讨sTRAIL水平与死亡风险之间的关系。方法采用双抗体夹心ELISA法检测50例脓毒症患者和20例健康志愿者血浆sTRAIL水平。用流式细胞仪检测单核细胞表面人类白细胞抗原DR的表达。记录有选择的生化参数，并在28天内监测患者的死亡率。结果脓毒症患者血浆sTRAIL水平显著低于健康对照组(16.9±8.3pg/ml比68.3±8.6pg/ml，P<；0.01)，存活28d组显著高于死亡组(19.4±9.8pg/ml比13.9±4.7pg/ml，P<；0.05)。单因素分析显示，血浆sTRAIL水平与单核细胞、淋巴细胞计数及HLADR表达水平呈正相关(r = 0.5，P<0.0 1；r = 0.3，P<0.0 5；r = 0.43，P<0.0 1)。STRAIL水平与APACHE II评分、BUN、年龄呈负相关(r = −分别为0.48，P<0.0 1；r = −为0.2 9，P<0.0 5；r = −为0.45，P<0.0 1)。多元线性回归分析显示，血浆可溶性TRAIL水平的预测因素为HLADR表达(P<；0.01)。结论感染性休克患者血浆sTRAIL水平降低与免疫性瘫痪和高死亡率有关。STRAIL可能被证明是一种潜在的免疫功能生物标志物，并预测脓毒症患者的生存。
Background Tumor necrosis factor related apoptosis inducing ligand (TRAIL) as a member of the TNF gene superfamily induces apoptosis primarily in tumor cells. TRAIL also plays an important role in the modulation of inflammatory responses, especially in the process of immune paralysis. The aim of the present study was to examine soluble TRAIL (sTRAIL) levels in septic patients in an attempt to explore the association between sTRAIL level and the risk of mortality. Methods Plasma sTRAIL levels were detected by ELISA in 50 septic patients and 20 healthy volunteers. HLA-DR expression in monocytes was detected by flow cytometry. Selective biochemical parameters were recorded, and patients were monitored in a 28-day period for mortality. Results The mean plasma sTRAIL level in septic patients was significantly lower than that in healthy controls (16.9±8.3 vs. 68.3±8.6 pg/ml, P<0.01), and was significantly higher in 28-day survivors than those in non-survivors (19.4±9.8 vs. 13.9±4.7 pg/ml, P<0.05). Univariate analysis indicated that plasma sTRAIL level was positively correlated with monocyte and lymphocyte counts and HLA-DR expression level (r = 0.5, P<0.01; r = 0.3, P<0.05; r = 0.43, P<0.01, respectively). STRAIL level was negatively correlated with APACHE II score, BUN and age (r = −0.48, P<0.01; r = −0.29, P<0.05; r = −0.45, P<0.01, respectively). Multiple linear regression analysis indicated that the predictor of plasma soluble TRAIL level was HLA-DR expression (P<0.01). Conclusion Low plasma sTRAIL levels were associated with immune paralysis and a high risk of mortality in patients with septic shock. sTRAIL may prove to be a potential biomarker of immune function and predict the survival of septic patients.
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DOI：
10.4049/jimmunol.1101180
发表时间：
2011-09-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Gurung P;Rai D;Condotta SA;Babcock JC;Badovinac VP;Griffith TS
通讯作者：
Griffith TS
影响因子：
120.7
作者：
Boomer, Jonathan S.;To, Kathleen;Chang, Kathy C.;Takasu, Osamu;Osborne, Dale F.;Walton, Andrew H.;Bricker, Traci L.;Jarman, Stephen D., II;Kreisel, Daniel;Krupnick, Alexander S.;Srivastava, Anil;Swanson, Paul E.;Green, Jonathan M.;Hotchkiss, Richard S.
通讯作者：
Hotchkiss, Richard S.
影响因子：
8.8
作者：
Levy, MM;Fink, MP;Ramsay, G
通讯作者：
Ramsay, G
影响因子：
2.8
作者：
Shaker, Olfat G.;El-Shehaby, Amal;Nabih, Mona
通讯作者：
Nabih, Mona
影响因子：
6.1
作者：
Liabeuf, Sophie;Barreto, Daniela V.;Massy, Ziad A.
通讯作者：
Massy, Ziad A.