Molecular analysis of two Ypt/Rab-related sequences isolated from soybean (Glycine max) DNA libraries
Molecular analysis of two Ypt/Rab-related sequences isolated from soybean (Glycine max) DNA libraries
复制标题
对从大豆 (Glycine max) DNA 文库中分离的两个 Ypt/Rab 相关序列进行分子分析
DOI：
10.1007/bf00039530
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发表时间：
1994
影响因子：
5.1
通讯作者：
C. Poulsen
中科院分区：
文献类型：
--
作者：
S. Borg;C. Poulsen
从根瘤和幼苗 cDNA 文库中，我们分离了两种 mRNA 的 cDNA 拷贝，它们源自基因 gmr1 和 gmr2，编码小 GTP 结合蛋白 Ypt/Rab 家族的成员。发现两种推导的蛋白质产物 GMR1 和 GMR2 几乎相同，仅在分析部分有四个氨基酸不同。这两种假定的蛋白质与之前描述的来自拟南芥的 ARA 小 GTP 酶有 79% 的相同性。因此，GMR 蛋白可能是 ARA 蛋白的对应物，并且可能在大豆中发挥相关的生物学功能。分离并分析了 gmr2 基因组序列。 Northern 和基于 cDNA 的 PCR 表达分析表明，gmr1 和 gmr2 基因在不同的植物器官中组成型表达，尽管在愈伤组织培养物中表达水平略高。将 gmr 序列分类为 Ypt/Rab 家族的亲属表明推导的 GMR 蛋白参与植物细胞中与囊泡运输相关的过程的控制。
From nodule and seedling cDNA libraries we isolated cDNA copies of two mRNAs, derived from the genes gmrl and gmr2, encoding members of the Ypt/Rab family of small GTP-binding proteins. Two deduced protein products, GMR1 and GMR2, were found to be nearly identical differing by only four amino acids in the analysed parts. The two putative proteins are 79% identical to the previously described ARA small GTPase from Arabidopsis thaliana. The GMR proteins may thus be the counterpart of the ARA protein and may perform a related biological function in Glycine max. The gmr2 genomic sequence was isolated and structurally analysed. Expression analyses by northern and cDNA-based PCR showed that the gmr1 and gmr2 genes are constitutively expressed in different plant organs, although at a slightly higher level in callus culture. The classification of the gmr sequences as relatives of the Ypt/Rab family suggests that the deduced GMR proteins are involved in control of processes related to vesicle trafficking in plant cells.
当肉毒杆菌外切酶 C3 ADP 核糖基化时，21 千道尔顿 G 蛋白的功能修饰。
影响因子：
5.3
作者：
Rubin,EJ;Gill,DM;Boquet,P;Popoff,MR
通讯作者：
Popoff,MR
SEC4 的突变分析表明了调节囊泡交通的循环机制。
DOI：
10.1002/j.1460-2075.1989.tb03560.x
发表时间：
1989
期刊：
The EMBO journal
影响因子：
--
作者：
Walworth,NC;Goud,B;Kabcenell,AK;Novick,PJ
通讯作者：
Novick,PJ