Genetic Mapping of Sexual Isolation Between E and Z Pheromone Strains of the European Corn Borer (Ostrinia nubilalis)
Genetic Mapping of Sexual Isolation Between E and Z Pheromone Strains of the European Corn Borer (Ostrinia nubilalis)
复制标题
欧洲玉米螟 (Ostrinia nubilalis) E 和 Z 信息素品系之间性隔离的遗传图谱
作者：
Erik B. Dopman;S. Bogdanowicz;R. Harrison
欧洲玉米螟（ECB）的E和Z信息素菌株提供了一个特殊的模式系统，检查性隔离的遗传基础。两个主要基因的差异导致雌性信息素产生和雄性行为反应的变化，这些信息素通信系统的组成部分已知对配偶识别和配偶选择很重要。ECB菌株在形态上是不可区分的，等位酶和DNA序列变异的调查显示，只有一个单一的性连锁位点，Tpi显着的等位基因频率差异。在这里，我们提出了一个详细的遗传连锁图ECB使用AFLP和微卫星标记和地图的因素负责信息素生产（Pher）和男性的反应（Resp）。我们的图谱覆盖1697 cM，并确定了ECB中的所有31个连锁群。Resp和Tpi都映射到Z（性）染色体，但这些标记之间的距离（>20 cM）反驳了Tpi的变异模式可以通过与这个“物种形成基因”紧密连锁来解释的假设。然而，我们表明，通过分析标记密度，Tpi位于低重组的区域，并建议第二个Z-连锁生殖障碍可能是负责的起源和/或持久性的分化Tpi。
The E and Z pheromone strains of the European corn borer (ECB) provide an exceptional model system for examining the genetic basis of sexual isolation. Differences at two major genes account for variation in female pheromone production and male behavioral response, components of the pheromone communication system known to be important for mate recognition and mate choice. Strains of ECB are morphologically indistinguishable, and surveys of allozyme and DNA sequence variation have revealed significant allele frequency differences at only a single sex-linked locus, Tpi. Here we present a detailed genetic linkage map of ECB using AFLP and microsatellite markers and map the factors responsible for pheromone production (Pher) and male response (Resp). Our map covers 1697 cM and identifies all 31 linkage groups in ECB. Both Resp and Tpi map to the Z (sex) chromosome, but the distance between these markers (>20 cM) argues against the hypothesis that patterns of variation at Tpi are explained by tight linkage to this “speciation gene.” However, we show, through analysis of marker density, that Tpi is located in a region of low recombination and suggest that a second Z-linked reproductive barrier could be responsible for the origin and/or persistence of differentiation at Tpi.
影响因子：
4
作者：
Aquadro, CF
通讯作者：
Aquadro, CF