Expression of prostaglandin E receptor subtype EP4 in conjunctival epithelium of patients with ocular surface disorders: case-control study.
Expression of prostaglandin E receptor subtype EP4 in conjunctival epithelium of patients with ocular surface disorders: case-control study.
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DOI：
10.1136/bmjopen-2012-001330
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发表时间：
2012
期刊：
影响因子：
2.9
通讯作者：
Kinoshita S
中科院分区：
文献类型：
--
作者：
Ueta M;Sotozono C;Yamada K;Yokoi N;Inatomi T;Kinoshita S
目的 证实Stevens-Johnson综合征/中毒性表皮坏死松解症（SJS/TEN）和眼部瘢痕性类天疱疮（OCP）患者结膜中PTGER4 mRNA的下调，并检测其EP4蛋白在各种眼表疾病患者结膜上皮中的表达。病例对照研究。我们对 SJS/TEN 和 OCP 患者的结膜组织切片中的 PTGER4 mRNA 进行定量逆转录 PCR (RT-PCR) 分析，以确认 PTGER4 mRNA 表达的下调。我们还对其他眼表疾病中的 EP4 进行了免疫组织学分析。结膜组织取自因化学眼烧伤、亚急性 SJS/TEN 或慢性 SJS/TEN、慢性 OCP、严重移植物抗宿主病 (GVHD) 而接受眼表手术重建的患者，以及通过切除炎性结膜治疗的蚕蚀性溃疡患者。通过定量RT-PCR测定和免疫组织学方法评估PTGER4 mRNA和EP4蛋白的表达。 SJS 和 OCP 患者的结膜组织中 PTGER4 mRNA 显着低于对照结膜松弛样本。在化学性眼烧伤患者的结膜上皮和结膜松弛症患者的对照结膜上皮中检测到 EP4 蛋白。它的表达在蚕蚀性溃疡患者的结膜上皮中存在差异。我们没有在亚急性 SJS/TEN、严重 GVHD、慢性 SJS/TEN 或 OCP 患者的结膜上皮中检测到 EP4 免疫反应性。 OCP 或 SJS/TEN 患者结膜上皮中 EP4 表达的强烈下调可能归因于眼表炎症。
To confirm the downregulation of PTGER4 mRNA in the conjunctiva of Stevens-Johnson syndrome/toxic epidermal necrolysis (SJS/TEN) and ocular cicatricial pemphigoid (OCP) patients and to examine the expression of its EP4 protein in the conjunctival epithelium of patients with various ocular surface disorders. Case-control study. We performed quantitative reverse transcription-PCR (RT-PCR) analysis of PTGER4 mRNA in conjunctival tissue sections from patients with SJS/TEN and OCP to confirm the downregulation of PTGER4 mRNA expression. We also analysed EP4 immunohistologically in other ocular surface disorders. Conjunctival tissues were obtained from patients undergoing surgical reconstruction of the ocular surface due to chemical eye burns, subacute SJS/TEN or chronic SJS/TEN, chronic OCP, severe graft versus host disease (GVHD) and from patients with Mooren's ulcers treated by resection of the inflammatory conjunctiva. The expression of PTGER4 mRNA and EP4 protein assessed by quantitative RT-PCR assay and immunohistological methods. PTGER4 mRNA was significantly lower in conjunctival tissues from SJS and OCP patients than in the control conjunctivochalasis samples. EP4 protein was detected in conjunctival epithelium from patients with chemical eye burn and in control conjunctival epithelium from patients with conjunctivochalasis. Its expression varied in conjunctival epithelium from patients with Mooren's ulcer. We did not detect EP4 immunoreactivity in conjunctival epithelium from patients with subacute SJS/TEN, severe GVHD, chronic SJS/TEN or OCP. The strong downregulation of EP4 expression in conjunctival epithelium from patients with OCP or SJS/TEN may be attributable to ocular surface inflammation.
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影响因子：
82.9
作者：
Yao, Chengcan;Sakata, Daiji;Narumiya, Shuh
通讯作者：
Narumiya, Shuh
影响因子：
4.4
作者：
Shiraishi, Hiroshi;Yoshida, Hideyuki;Yoshimura, Akihiko
通讯作者：
Yoshimura, Akihiko
DOI：
10.1152/ajpgi.1997.272.3.g681
发表时间：
1997-03-01
影响因子：
4.5
作者：
Morimoto, K;Sugimoto, Y;Ichikawa, A
通讯作者：
Ichikawa, A
影响因子：
15.9
作者：
Kabashima, K;Saji, T;Narumiya, S
通讯作者：
Narumiya, S
影响因子：
--
作者：
Matsuoka T;Narumiya S
通讯作者：
Narumiya S