Sympathetic and sensory innervation of the urinary tract in young adult and aged rats: a semi-quantitative histochemical and immunohistochemical study
Sympathetic and sensory innervation of the urinary tract in young adult and aged rats: a semi-quantitative histochemical and immunohistochemical study
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年轻成年和老年大鼠泌尿道的交感神经和感觉神经支配：半定量组织化学和免疫组织化学研究
DOI：
10.1007/bf00157961
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
R. Santer
中科院分区：
文献类型：
--
作者：
A. L. Warburton;R. Santer
摘要通过乙醛酸诱导的荧光检测去甲肾上腺素，并通过使用抗酪氨酸羟化酶 (TH) 和神经肽 Y (NPY) 的抗血清进行免疫荧光检查，对年轻成年（4 个月）和老年（24 个月以上）大鼠的尿路交感神经支配进行了检查。还对已知存在于盆腔感觉神经中的降钙素基因相关肽（CGRP）进行了免疫染色。神经密度的半定量估计是由支配输尿管、膀胱和尿道以及尿路脉管系统的平滑肌组织的去甲肾上腺素能和肽能纤维进行的。在老年大鼠中，神经支配的总体模式保持不变。然而，除膀胱脉管系统外，去甲肾上腺素能神经支配的密度下降，组织荧光的强度也下降。 TH 和 NPY 免疫荧光观察到类似的结果模式。结果提供了老年大鼠泌尿道交感神经控制减弱的证据。老年动物中 CGRP 免疫反应性神经的模式和密度没有变化，表明盆腔内脏感觉神经支配对年龄增长的影响更具抵抗力。
SummaryThe sympathetic innervation of the urinary tract of young adult (4 months) and aged (24+ months) rats has been examined by glyoxylic acid-induced fluorescence for the detection of noradrenaline and by immunofluorescence using antisera against tyrosine hydroxylase (TH) and neuropeptide Y (NPY). Immunostaining for calcitonin gene-related peptide (CGRP), known to be present in pelvic sensory nerves, was also performed. Semi-quantitative estimations of nerve densities were made of noradrenergic and peptidergic fibres innervating the smooth musculature of the ureter, bladder and urethra, and of the urinary tract vasculature. In the aged rats the overall patterns of innervation remained unchanged. However, with the exception of the vesical vasculature, the density of noradrenergic innervation decreased as did the intensity of histofluorescence. A similar pattern of results was observed by TH and NPY immunofluorescence. The results present evidence for a diminution in the sympathetic control of the urinary tract in aged rats. The pattern and density of CGRP-immunoreactive nerves was unchanged in the aged animals suggesting that pelvic visceral sensory innervation is more resistant to the effects of advancing age.
大鼠肾脏中对神经肽 Y 和血管活性肠肽具有免疫反应性的去甲肾上腺素能神经末梢的鉴定。
DOI：
10.1002/aja.1001840303
发表时间：
1989
期刊：
The American journal of anatomy
影响因子：
--
作者：
Knight,DS;Fabre,RD;Beal,JA
通讯作者：
Beal,JA