Metabolomics reveals the heterogeneous secretome of two entomopathogenic fungi to ex vivo cultured insect tissues.
Metabolomics reveals the heterogeneous secretome of two entomopathogenic fungi to ex vivo cultured insect tissues.
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DOI：
10.1371/journal.pone.0070609
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Hughes DP
中科院分区：
文献类型：
--
作者：
de Bekker C;Smith PB;Patterson AD;Hughes DP
真菌昆虫病原体在昆虫宿主感染的不同阶段依赖于细胞异质性。它们的致病性是通过次级代谢产物的分泌表现出来的，这意味着这组环境重要真菌的感染生活史可以利用代谢组学来揭示。代谢组学分析与离体昆虫组织培养相结合表明，常用作生物农药的绿僵菌属和白僵菌属的两种多面手分离株在感染性和寄生性生长期间采用显著不同的次级代谢产物阵列。这也揭示了这两种真菌通过在本研究中测试的昆虫组织上可区分的代谢物分泌而表现出组织特异性策略。除了显示这两种昆虫病原体的重要异质性外，这项研究还发现了几种以前没有描述过的新型destruxins和beauverolides，很可能是因为以前的调查没有使用昆虫组织作为培养系统。白僵菌在遇到活的昆虫组织时会分泌这些环状缩肽，而绿僵菌则主要在死的组织上分泌。这意味着，虽然这些真菌采用类似的策略，当涉及到昆虫发病机制，有最肯定的是在分子水平上的显着差异，值得研究。
Fungal entomopathogens rely on cellular heterogeneity during the different stages of insect host infection. Their pathogenicity is exhibited through the secretion of secondary metabolites, which implies that the infection life history of this group of environmentally important fungi can be revealed using metabolomics. Here metabolomic analysis in combination with ex vivo insect tissue culturing shows that two generalist isolates of the genus Metarhizium and Beauveria, commonly used as biological pesticides, employ significantly different arrays of secondary metabolites during infectious and saprophytic growth. It also reveals that both fungi exhibit tissue specific strategies by a distinguishable metabolite secretion on the insect tissues tested in this study. In addition to showing the important heterogeneous nature of these two entomopathogens, this study also resulted in the discovery of several novel destruxins and beauverolides that have not been described before, most likely because previous surveys did not use insect tissues as a culturing system. While Beauveria secreted these cyclic depsipeptides when encountering live insect tissues, Metarhizium employed them primarily on dead tissue. This implies that, while these fungi employ comparable strategies when it comes to entomopathogenesis, there are most certainly significant differences at the molecular level that deserve to be studied.
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影响因子：
2.3
作者：
Kuzma, M;Jegorov, A;Havlícek, V
通讯作者：
Havlícek, V
影响因子：
2.5
作者：
Donzelli, Bruno Giuliano Garisto;Krasnoff, Stuart B.;Gibson, Donna M.
通讯作者：
Gibson, Donna M.
影响因子：
3.2
作者：
Mnyone LL;Ng'habi KR;Mazigo HD;Katakweba AA;Lyimo IN
通讯作者：
Lyimo IN
影响因子：
--
作者：
Akbarian, Jamshid;Ghosta, Youbert;Safavi, Seyed Ali
通讯作者：
Safavi, Seyed Ali
DOI：
10.1039/p19800002878
发表时间：
1980-01-01
期刊：
JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1
影响因子：
--
作者：
GROVE, JF
通讯作者：
GROVE, JF