Age-related severity of focal ischemia in female rats is associated with impaired astrocyte function.
Age-related severity of focal ischemia in female rats is associated with impaired astrocyte function.
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DOI：
10.1016/j.neurobiolaging.2011.11.007
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发表时间：
2012-06
影响因子：
4.2
通讯作者：
Sohrabji F
中科院分区：
文献类型：
--
作者：
Lewis DK;Thomas KT;Selvamani A;Sohrabji F
在中年雌性大鼠中，与年轻雌性相比，局灶性缺血导致更大的皮质梗死。为了确定中风诱导的中年雌性细胞毒性是否与星形胶质细胞功能受损有关，我们从年轻和中年雌性大鼠的缺血皮质中收获并培养了星形胶质细胞。与年轻女性星形胶质细胞相比，中年星形胶质细胞从培养基中清除谷氨酸明显减少。此外，来自中年女性星形胶质细胞的星形胶质细胞条件培养基诱导外周血单核细胞（PBMCs）的更大迁移，并表达更高水平的化学引诱物巨噬细胞炎症蛋白-1 （MIP-1）。中年星形胶质细胞也能诱导神经祖细胞（npc）更大的迁移，但其促进神经祖细胞神经元分化的能力与年轻星形胶质细胞相似。在雄性动物中，皮层梗死体积在年轻和中年动物中相似，没有观察到星形胶质细胞功能的年龄相关损伤。这些研究表明，老化的星形胶质细胞可能通过谷氨酸清除效率低下和细胞因子产生增强而直接导致梗死严重程度，并提示老年女性中风治疗的细胞靶点。
In middle-aged female rats, focal ischemia leads to a larger cortical infarction as compared with younger females. To determine if stroke-induced cytotoxicity in middle-aged females was associated with impaired astrocyte function, astrocytes were harvested and cultured from the ischemic cortex of young and middle-aged female rats. Middle-aged astrocytes cleared significantly less glutamate from media as compared with young female astrocytes. Furthermore, astrocyte-conditioned media from middle-aged female astrocytes induced greater migration of peripheral blood monocyte cells (PBMCs) and expressed higher levels of the chemoattractant macrophage inflammatory protein-1 (MIP-1). Middle-aged astrocytes also induced greater migration of neural progenitor cells (NPCs), however, their ability to promote neuronal differentiation of neural progenitor cells was similar to young astrocytes. In males, where cortical infarct volume is similar in young and middle-aged animals, no age-related impairment was observed in astrocyte function. These studies show that the aging astrocyte may directly contribute to infarct severity by inefficient glutamate clearance and enhanced cytokine production and suggest a cellular target for improved stroke therapy among older females.
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影响因子：
4.7
作者：
BENVENISTE, H;DREJER, J;DIEMER, NH
通讯作者：
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影响因子：
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通讯作者：
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