lncRNA profiling in NCI-H292 cells after stimulation with Dermatophagoides farinae extracts.
lncRNA profiling in NCI-H292 cells after stimulation with Dermatophagoides farinae extracts.
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用粉尘螨提取物刺激后 NCI-H292 细胞中的 lncRNA 分析
DOI：
10.1177/0394632017750997
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发表时间：
2018-01
影响因子：
3.5
通讯作者：
Cui Y
中科院分区：
文献类型：
--
作者：
Wang L;Zhou Y;Cui Y
气道上皮细胞是抵抗空气中过敏原的第一道防线。当培养时，上皮细胞可以暴露于各种过敏原，为研究过敏性疾病提供了理想的模型。本研究旨在描述暴露于屋尘螨过敏原的气道上皮细胞中长非编码（lnc）RNA的特征，这些RNA可以调节基因表达并在不同的细胞过程中发挥功能。将NCI-H292细胞暴露于屋尘螨提取物24小时。在暴露和未暴露的细胞中分析RNA表达。粉尘螨提取物刺激NCI-H292细胞后，有270条lncRNA差异表达（变化倍数≥ 2，P < 0.05）。此外，119 lncRNA和22信使RNA共表达。Gene Ontology分析表明，这些上调和下调的lncRNA与生物学过程、细胞成分和分子功能有关。通过对重要调控信号通路的生物信息学分析，我们发现这些lncRNA可能靶向16条基因通路，包括糖酵解、轴突导向、ErbB信号通路和丝裂原活化蛋白激酶（MAPK）信号通路。粉尘螨提取物刺激后NCI-H292细胞中差异调节的lncRNA及其靶基因通路的鉴定可以为过敏的病因学和发病机制提供见解。
Airway epithelium cells are the first line of defense against airborne allergens. When cultured, epithelial cells can be exposed to various allergens, providing an ideal model to investigate allergic disorders. This study sought to characterize the profile of long noncoding (lnc) RNAs, which can regulate gene expression and exert functions in diverse cellular processes, in airway epithelial cells exposed to house dust mite allergens. NCI-H292 cells were exposed to house dust mite extract for 24 h. RNA expression was profiled in exposed and unexposed cells. There were 270 lncRNAs that were differentially expressed (fold change ≥ 2, P < 0.05) in NCI-H292 cells after stimulation with Dermatophagoides farinae (house dust mite) extracts. Furthermore, 119 lncRNAs and 22 messenger RNAs were co-expressed. Gene Ontology analysis showed that these under-regulated and up-regulated lncRNAs were associated with biological process, cellular component, and molecular function. After bioinformatic analysis of significantly regulated signaling pathways, we found these lncRNAs may target 16 gene pathways, including glycolysis, axon guidance, ErbB signaling, and mitogen-activated protein kinases (MAPK) signaling. The identification of differentially regulated lncRNAs in NCI-H292 cells after stimulation with Dermatophagoides farinae extracts, as well as their target gene pathways, can provide insight to the etiology and pathogenesis of allergy.
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影响因子：
5.8
作者：
Xu YD;Cui JM;Wang Y;Yin LM;Gao CK;Liu YY;Yang YQ
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影响因子：
6.1
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影响因子：
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Tsitsiou, Eleni;Williams, Andrew E.;Lindsay, Mark A.
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DOI：
10.1164/rccm.200602-189oc
发表时间：
2006-11-01
影响因子：
24.7
作者：
Barbato, Angelo;Turato, Graziella;Saetta, Marina
通讯作者：
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