The early asthmatic response is associated with glycolysis, calcium binding and mitochondria activity as revealed by proteomic analysis in rats.
The early asthmatic response is associated with glycolysis, calcium binding and mitochondria activity as revealed by proteomic analysis in rats.
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大鼠蛋白质组分析显示，早期哮喘反应与糖酵解、钙结合和线粒体活性有关
DOI：
10.1186/1465-9921-11-107
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发表时间：
2010-08-06
影响因子：
5.8
通讯作者：
Yang YQ
中科院分区：
文献类型：
--
作者：
Xu YD;Cui JM;Wang Y;Yin LM;Gao CK;Liu YY;Yang YQ
背景过敏性哮喘患者吸入过敏原导致早期哮喘反应(EAR)，其特征是在几分钟内开始急性呼吸道阻塞。耳朵是过敏性哮喘病理进展的最早指标。由于哮喘的分子机制尚未完全明确，本研究将有助于更好地了解哮喘的分子基础。方法采用2-DE/MS蛋白质组学技术，检测哮喘大鼠在耳部发育过程中肺组织蛋白质表达模式的变化，以了解哮喘发病的分子基础。利用PPI Spider和KEGG Spider对蛋白质组数据进行生物信息学分析，以探讨其可能的分子机制。在这44个蛋白质点中，42个对应于36个独特的蛋白质，通过质谱学成功鉴定。在随后的生物信息学分析中，基因本体论分类、蛋白质-蛋白质相互作用网络和生物途径探索表明，所鉴定的蛋白质主要参与糖酵解、钙结合和线粒体活性。免疫印迹和半定量RT-PCR证实了5种蛋白表达的变化，进一步支持了我们的蛋白质组和生物信息学分析。结论我们的结果揭示了过敏原诱导的哮喘大鼠EAR与糖酵解、钙结合和线粒体活性有关，这可能建立了一个功能网络，其中钙结合可能在促进哮喘进展中发挥核心作用。
BackgroundThe inhalation of allergens by allergic asthmatics results in the early asthmatic response (EAR), which is characterized by acute airway obstruction beginning within a few minutes. The EAR is the earliest indicator of the pathological progression of allergic asthma. Because the molecular mechanism underlying the EAR is not fully defined, this study will contribute to a better understanding of asthma.MethodsIn order to gain insight into the molecular basis of the EAR, we examined changes in protein expression patterns in the lung tissue of asthmatic rats during the EAR using 2-DE/MS-based proteomic techniques. Bioinformatic analysis of the proteomic data was then performed using PPI Spider and KEGG Spider to investigate the underlying molecular mechanism.ResultsIn total, 44 differentially expressed protein spots were detected in the 2-DE gels. Of these 44 protein spots, 42 corresponded to 36 unique proteins successfully identified using mass spectrometry. During subsequent bioinformatic analysis, the gene ontology classification, the protein-protein interaction networking and the biological pathway exploration demonstrated that the identified proteins were mainly involved in glycolysis, calcium binding and mitochondrial activity. Using western blot and semi-quantitative RT-PCR, we confirmed the changes in expression of five selected proteins, which further supports our proteomic and bioinformatic analyses.ConclusionsOur results reveal that the allergen-induced EAR in asthmatic rats is associated with glycolysis, calcium binding and mitochondrial activity, which could establish a functional network in which calcium binding may play a central role in promoting the progression of asthma.
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影响因子：
11.6
作者：
Graham, James W. A.;Williams, Thomas C. R.;Sweetlove, Lee J.
通讯作者：
Sweetlove, Lee J.
影响因子：
46.9
作者：
Ozawa, T;Sako, Y;Umezawa, Y
通讯作者：
Umezawa, Y
影响因子：
--
作者：
KROEGER, E;STEPHENS, NL
通讯作者：
STEPHENS, NL
影响因子：
3.7
作者：
Hermani, A;De Servi, B;Mayer, D
通讯作者：
Mayer, D
影响因子：
7.8
作者：
Ernster, L;Schatz, G
通讯作者：
Schatz, G