Hesperidin, a citrus flavonoid, protects against l-methionine-induced hyperhomocysteinemia by abrogation of oxidative stress, endothelial dysfunction and neurotoxicity in Wistar rats.
Hesperidin, a citrus flavonoid, protects against l-methionine-induced hyperhomocysteinemia by abrogation of oxidative stress, endothelial dysfunction and neurotoxicity in Wistar rats.
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DOI：
10.1080/13880209.2016.1231695
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发表时间：
2017-12
影响因子：
3.8
通讯作者：
Diwan PV
中科院分区：
文献类型：
--
作者：
Hemanth Kumar B;Dinesh Kumar B;Diwan PV
橙皮苷（HSP）是一种在柑橘类水果中发现的黄烷糖苷配基，具有抗氧化、抗炎和神经保护作用。目的：探讨热休克蛋白（HSP）对L-蛋氨酸诱导的大鼠高同型半胱氨酸血症（HHcy）的保护作用。材料与方法：雄性Wistar大鼠随机分为DMSO、L-蛋氨酸、HSP（25、50和100 mg/kg）、HSP本身（100 mg/kg）和多奈哌齐（0.1 mg/kg）7组。通过口服l-蛋氨酸（1.7g/kg）32天诱导HHcy。从研究的第14天开始，HSP（25、50和100 mg/kg）和多奈哌齐经口给予L-甲硫氨酸处理的大鼠。采用Morris水迷宫（MWM）和视频跟踪Y-迷宫检测HHcy所致大鼠认知功能障碍（28 ~ 32天）。评估血清和脑中HHcy的不同生物标志物和血管反应性，并进行组织病理学（胸主动脉和脑）。结果如下：HSP（100 mg/kg）治疗的L-蛋氨酸处理大鼠表现出显着的（p < 0.001）剂量依赖性活动和减少行为缺陷，脑乙酰胆碱酯酶（25.99 ± 2.36对10.73 ± 1.26 μ mol/mg），脑脂质过氧化（15.25 ± 1.65 vs 6.18 ± 0.74 nM/mg）、血清同型半胱氨酸（Hcy）（22.37 ± 0.30 vs 11.01 ± 1.01 μg/mL）和血清胆固醇（182.7 ± 2.15 vs 101.5 ± 2.76 mg/dL）以及脑抗氧化剂水平升高。HSP通过消除L-蛋氨酸对乙酰胆碱诱导的内皮依赖性舒张的作用显著（p < 0.001）降低内皮功能障碍（艾德），并增加血清亚硝酸盐和血管一氧化氮的生物利用度，沿着组织学畸变的恢复。结论：HSP通过减轻氧化应激、艾德和神经毒性对HHcy具有保护作用。
Context: Hesperidin (HSP), a flavanoglycone found in citrus fruits, has antioxidant, anti-inflammatory and neuroprotective properties. Objective: This study evaluates the protective effect of HSP on l-methionine-induced hyperhomocysteinemia (HHcy) in rats. Materials and methods: Male Wistar rats were randomly divided into seven groups as DMSO, l-methionine, HSP (25, 50 and 100 mg/kg), HSP-per se (100 mg/kg) and donepezil (0.1 mg/kg). HHcy was induced by oral administration of l-methionine (1.7 g/kg) for 32 days. From the 14th day of study HSP (25, 50 and 100 mg/kg) and donepezil was administered orally to l-methionine-treated rats. Cognitive impairment induced by HHcy was determined using the Morris water maze (MWM) and Y-maze on video tracking system (28th–32nd day). Different biomarkers of HHcy in serum and brain and vascular reactivity were evaluated and histopathology (thoracic aorta and brain) was done. Results: HSP (100 mg/kg) treatment in l-methionine-treated rats exhibited significant (p < 0.001) dose-dependent activity and reduced behavioural deficits, brain acetylcholinesterase (25.99 ± 2.36 versus 10.73 ± 1.26 μmoles/mg), brain lipid peroxidation (15.25 ± 1.65 versus 6.18 ± 0.74 nM/mg), serum homocysteine (Hcy) (22.37 ± 0.30 versus 11.01 ± 1.01 μg/mL) and serum cholesterol (182.7 ± 2.15 versus 101.5 ± 2.76 mg/dL) and increased brain antioxidant levels. HSP significantly (p < 0.001) reduced endothelial dysfunction (ED) by abolishing the effect of l-methionine on acetylcholine-induced endothelial-dependent relaxation and increased serum nitrite and vascular nitric oxide bioavailability along with the restoration of histological aberrations. Conclusion: HSP exerts a protective effect on HHcy by abrogating oxidative stress, ED and neurotoxicity.
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影响因子：
5.8
作者：
ELLMAN, GL;COURTNEY, KD;FEATHERSTONE, RM
通讯作者：
FEATHERSTONE, RM
影响因子：
15.9
作者：
Landmesser, U;Dikalov, S;Harrison, DG
通讯作者：
Harrison, DG
影响因子：
2.7
作者：
Kumar, Puneet;Kumar, Anil
通讯作者：
Kumar, Anil
DOI：
10.1161/01.atv.0000189559.87328.e4
发表时间：
2005-12-01
影响因子：
8.7
作者：
Jiang, XH;Yang, F;Wang, H
通讯作者：
Wang, H
影响因子：
3.9
作者：
Maher, Pamela
通讯作者：
Maher, Pamela