Limited impacts of thermoneutral housing on bone morphology and mechanical properties in growing female mice exposed to external loading and raloxifene treatment.
Limited impacts of thermoneutral housing on bone morphology and mechanical properties in growing female mice exposed to external loading and raloxifene treatment.
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DOI：
10.1016/j.bone.2021.115889
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发表时间：
2021-05
期刊：
影响因子：
4.1
通讯作者：
Wallace JM
中科院分区：
文献类型：
--
作者：
Tastad CA;Kohler R;Wallace JM
体温调节是一个重要因素，可能对临床前研究结果产生生理后果。已经证明，将小鼠简单地置于热中性温度下可以防止生长中小鼠典型的松质骨丢失。在这项研究中，通过非侵入性胫骨负荷诱导雌性小鼠的活性组织形成，并与雷洛昔芬治疗相结合，以评估温度是否可以增强其对骨形态和机械性能的综合影响。假设通过去除正常实验室小鼠所处的冷应激，代谢增强将允许暴露于胫骨负荷和雷洛昔芬组合的小鼠的结构和机械改善。用雷洛昔芬处理10周龄雌性C57 BL/6 J小鼠，使其经受胫骨负荷至2050 μ s的最大拉伸应力，并在热中性条件（32 ℃）下饲养6周。我们通过显微计算机断层扫描（μCT）研究了骨形态，通过四点弯曲和断裂韧性测试研究了力学性能。结果证实了先前的工作，显示了外部负荷和雷洛昔芬的综合作用，这导致了更大的改善，在大多数属性比任何一个单独的治疗。与该假设相反，温度对体重、总体骨大小和骨小梁结构有适度影响，大多数影响是有害的。热中性对机械完整性或断裂韧性没有影响。在大多数情况下，基于温度的影响的幅度不如RAL处理或负载稳健。
Thermoregulation is an important factor that could have physiological consequences on pre-clinical research outcomes. Simply housing mice at thermoneutral temperature has been shown to prevent the well-established loss of cancellous bone that is typical in growing mice. In this study, active tissue formation was induced by non-invasive tibial loading in female mice and combined with raloxifene treatment to assess whether temperature could enhance their combined effects on bone morphology and mechanical properties. It was hypothesized that by removing the cold stress under which normal lab mice are housed, a metabolic boost would allow for further architectural and mechanical improvements in mice exposed to a combination of tibial loading and raloxifene. Ten-week old female C57BL/6J mice were treated with raloxifene, underwent tibial loading to a maximum tensile stress of 2050 με, and were housed in thermoneutral conditions (32°C) for 6 weeks. We investigated bone morphology through microcomputed tomography (μCT), mechanical properties via four-point bending, and fracture toughness testing. Results confirmed previous work showing a combined effect of external loading and raloxifene which led to greater improvements in most properties than either individual treatment. Counter to the hypothesis, temperature had modest effects on body weight, overall bone size, and trabecular architecture, and most effects were detrimental. Thermoneutrality had no impact on mechanical integrity or fracture toughness. In most cases, the magnitude of temperature-based effects were less robust than either RAL treatment or loading.
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影响因子：
4.1
作者：
Gallant, Maxime A.;Brown, Drew M.;Hammond, Max;Wallace, Joseph M.;Du, Jiang;Deymier-Black, Alix C.;Almer, Jonathan D.;Stock, Stuart R.;Allen, Matthew R.;Burr, David B.
通讯作者：
Burr, David B.
DOI：
10.1007/s00198-016-3634-3
发表时间：
2016-10
期刊：
Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA
影响因子：
--
作者：
Iwaniec UT;Philbrick KA;Wong CP;Gordon JL;Kahler-Quesada AM;Olson DA;Branscum AJ;Sargent JL;DeMambro VE;Rosen CJ;Turner RT
通讯作者：
Turner RT
在鼠骨细胞中去除或截断连接蛋白43改变了胫骨的皮质几何形状，纳米级形态和组织力学。
影响因子：
4.1
作者：
Hammond MA;Berman AG;Pacheco-Costa R;Davis HM;Plotkin LI;Wallace JM
通讯作者：
Wallace JM
影响因子：
3.7
作者：
Berman AG;Clauser CA;Wunderlin C;Hammond MA;Wallace JM
通讯作者：
Wallace JM
影响因子：
6.2
作者：
Fuchs, RK;Bauer, JJ;Snow, CM
通讯作者：
Snow, CM