Structural and Mechanical Improvements to Bone Are Strain Dependent with Axial Compression of the Tibia in Female C57BL/6 Mice.
Structural and Mechanical Improvements to Bone Are Strain Dependent with Axial Compression of the Tibia in Female C57BL/6 Mice.
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DOI：
10.1371/journal.pone.0130504
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Wallace JM
中科院分区：
文献类型：
--
作者：
Berman AG;Clauser CA;Wunderlin C;Hammond MA;Wallace JM
在小鼠胫骨的轴向加载模型中，应变诱导的骨适应已经得到了很好的研究。然而，这些研究的大多数结果仅限于骨骼结构的变化，并没有探索这些变化的力学含义。在这里，我们使用靶向胫骨加载的小鼠模型研究了骨对三种应变水平的力学和形态适应性。我们假设负荷会以剂量依赖的方式增加骨骼结构和改善皮质力学性能。将8周龄雌性C57BL/6小鼠的右侧胫骨压缩2周，最大压力分别为8.8N、10.6N或12.4N(应变校准法确定在骨干中部前内侧区域产生1700με、2050με或2400με的骨膜应变)，而左侧作为非加载对照。加载后，通过显微计算机断层扫描和四点弯曲对骨结构和皮质力学完整性进行体外分析。结果表明，负荷以剂量依赖的方式改善了骨结构，并在2050με时改善了力学结果。加载到2050年με在皮质和松质层区域都产生了强烈和令人信服的地层响应。此外，骨干的结构和组织水平的强度和能量耗散都受到正向影响。加载到最高应变水平也会导致皮质区和松质区快速而强健的骨形成。然而，这些改进是以一半动物的编织骨骼反应为代价的。加载到最低应变水平对骨结构几乎没有影响，也没有影响结构或组织水平的机械性能。骨小梁体积分数以及力-位移和应力-应变曲线的屈服前区域存在潜在的全身性影响。未来的研究将集中在适度的负荷水平，这在很大程度上有利于皮质/松质骨结构和皮质机械功能。
Strain-induced adaption of bone has been well-studied in an axial loading model of the mouse tibia. However, most outcomes of these studies are restricted to changes in bone architecture and do not explore the mechanical implications of those changes. Herein, we studied both the mechanical and morphological adaptions of bone to three strain levels using a targeted tibial loading mouse model. We hypothesized that loading would increase bone architecture and improve cortical mechanical properties in a dose-dependent fashion. The right tibiae of female C57BL/6 mice (8 week old) were compressively loaded for 2 weeks to a maximum compressive force of 8.8N, 10.6N, or 12.4N (generating periosteal strains on the anteromedial region of the mid-diaphysis of 1700 με, 2050 με, or 2400 με as determined by a strain calibration), while the left limb served as an non-loaded control. Following loading, ex vivo analyses of bone architecture and cortical mechanical integrity were assessed by micro-computed tomography and 4-point bending. Results indicated that loading improved bone architecture in a dose-dependent manner and improved mechanical outcomes at 2050 με. Loading to 2050 με resulted in a strong and compelling formation response in both cortical and cancellous regions. In addition, both structural and tissue level strength and energy dissipation were positively impacted in the diaphysis. Loading to the highest strain level also resulted in rapid and robust formation of bone in both cortical and cancellous regions. However, these improvements came at the cost of a woven bone response in half of the animals. Loading to the lowest strain level had little effect on bone architecture and failed to impact structural- or tissue-level mechanical properties. Potential systemic effects were identified for trabecular bone volume fraction, and in the pre-yield region of the force-displacement and stress-strain curves. Future studies will focus on a moderate load level which was largely beneficial in terms of cortical/cancellous structure and cortical mechanical function.
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影响因子：
4.1
作者：
Lynch, Maureen E.;Main, Russell P.;Xu, Qian;Schmicker, Thomas L.;Schaffler, Mitchell B.;Wright, Timothy M.;van der Meulen, Marjolein C. H.
通讯作者：
van der Meulen, Marjolein C. H.
影响因子：
3.3
作者：
Lynch, Maureen E.;Main, Russell P.;van der Meulen, Marjolein C. H.
通讯作者：
van der Meulen, Marjolein C. H.
影响因子：
4.1
作者：
Birkhold, Annette I.;Razi, Hajar;Willie, Bettina M.
通讯作者：
Willie, Bettina M.
影响因子：
2.4
作者：
Patel, Tarpit K.;Brodt, Michael D.;Silva, Matthew J.
通讯作者：
Silva, Matthew J.
影响因子：
2.2
作者：
Rho, JY;Kuhn-Spearing, L;Zioupos, P
通讯作者：
Zioupos, P