Forms of Selenium Affect its Transport, Uptake and Glutathione Peroxidase Activity in the Caco-2 Cell Model
Forms of Selenium Affect its Transport, Uptake and Glutathione Peroxidase Activity in the Caco-2 Cell Model
复制标题
硒的形式影响 Caco-2 细胞模型中的转运、摄取和谷胱甘肽过氧化物酶活性
DOI：
10.1007/s12011-012-9395-8
复制
发表时间：
2012-03
影响因子：
3.9
通讯作者：
Fu, Linglin
中科院分区：
文献类型：
--
作者：
Wang, Yanbo;Fu, Linglin
本实验旨在研究硒的化学形式（亚硒酸钠、纳米硒和硒代蛋氨酸）在Caco-2细胞模型中对转运、摄取和谷胱甘肽过氧化物酶（GSH-Px）活性的影响。在Caco-2细胞单层上分别沿两个方向（顶侧[AP]至基底侧[BL]和基底侧至AP）转运和摄取不同形式的Se（0.1 μmol l−1）2 h，并计算表观渗透系数（Papp）、转运效率和摄取效率。在本研究中，三种形式的硒的运输和吸收的时间依赖于AP到BL和BL到AP方向。2 h时，硒代蛋氨酸和纳米硒的转运效率均高于亚硒酸钠（P< 0.05）。纳米硒组细胞摄取效率最高（P< 0.05），亚硒酸钠组和硒代蛋氨酸组细胞摄取效率差异有统计学意义（P< 0.05）。在AP-BL和BL-AP中，亚硒酸钠的Papp值均低于硒代蛋氨酸和纳米硒（P< 0.05）。但GSH-Px活性无显著差异。这些结果表明，硒在Caco-2细胞中的转运效率因其化学形态而异，这可能与硒在细胞中的转运和摄取差异有关。
The experiment was designed to investigate the effect of selenium (Se) chemical forms (sodium selenite, selenium nanoparticle [nano-Se] and selenomethionine) on the transport, uptake and glutathione peroxidase (GSH-Px) activity in the Caco-2 cell model. The transport and uptake of different forms of Se (0.1 μmol l−1) across the Caco-2 cell monolayer were carried out in two directions (apical [AP] to basolateral [BL] and BL to AP) for 2 h, respectively, and the apparent permeability coefficient (Papp), transport efficiency and uptake efficiency were all calculated. In the present study, the transport and uptake of three forms of Se were time-dependent both in AP to BL and BL to AP directions. By the end of 2 h, the transport efficiencies of selenomethionine and nano-Se were higher than that of sodium selenite (P< 0.05). The highest uptake efficiency (P< 0.05) was observed in cells treated with nano-Se and significant difference (P< 0.05) was also observed between the cells incubated with sodium selenite and selenomethionine. As for thePapp, sodium selenite (P< 0.05) had the lowest values compared with that of selenomethionine and nano-Se, in both AP–BL and BL–AP. However, no significant differences were observed in GSH-Px activities. These results indicated that the efficiency of Se in the Caco-2 cells varied with its chemical forms, which might be associated with the differences in Se transport and uptake.
登录
查看更多内容
DOI：
10.1016/0005-2736(94)90121-x
发表时间：
1994-06
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
X. Alvarez-Hernandez;Margaret Smith;Jonathan Glass
通讯作者：
X. Alvarez-Hernandez;Margaret Smith;Jonathan Glass
影响因子：
4.4
作者：
P. Utterback;C. Parsons;I. Yoon;J. Butler
通讯作者：
P. Utterback;C. Parsons;I. Yoon;J. Butler
影响因子：
3.7
作者：
Desai, MP;Labhasetwar, V;Amidon, GL
通讯作者：
Amidon, GL
影响因子：
6
作者：
Combs, GF;Clark, LC;Turnbull, BW
通讯作者：
Turnbull, BW
影响因子：
11.2
作者：
J. Mohandas;J. J. Marshall-J.;G. Duggin;J. Horvath;D. Tiller
通讯作者：
J. Mohandas;J. J. Marshall-J.;G. Duggin;J. Horvath;D. Tiller