Chemical genomic-based pathway analyses for epidermal growth factor-mediated signaling in migrating cancer cells.
Chemical genomic-based pathway analyses for epidermal growth factor-mediated signaling in migrating cancer cells.
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DOI：
10.1371/journal.pone.0096776
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Imoto M
中科院分区：
文献类型：
--
作者：
Magi S;Saeki Y;Kasamatsu M;Tashiro E;Imoto M
为了探索调节肿瘤细胞迁移的信号通路的多样性和一致性，我们选择了三种在 EGF 处理后发生迁移的人类肿瘤细胞系。然后，我们量化了 15 种抑制剂对这些细胞系中由 EGF 刺激诱导的 9 种蛋白质的表达水平或磷酸化水平的影响。根据本研究获得的数据和化学生物学假设，我们推导了每个肿瘤细胞系的细胞迁移途径，然后进行比较。结果，我们发现 MEK/ERK 和 JNK/c-Jun 通路在所有三种迁移细胞系中均被激活。此外，仅在 EC109 细胞中发现 GSK-3 和 p38 调节 PI3K/Akt 通路，并且仅在 TT 细胞中发现 JNK 与 p38 和 Fos 相关通路串扰。总而言之，我们的分析系统可以轻松区分导致肿瘤细胞迁移的常见途径和细胞类型特异性途径。
To explore the diversity and consistency of the signaling pathways that regulate tumor cell migration, we chose three human tumor cell lines that migrated after treatment with EGF. We then quantified the effect of fifteen inhibitors on the levels of expression or the phosphorylation levels of nine proteins that were induced by EGF stimulation in each of these cell lines. Based on the data obtained in this study and chemical-biological assumptions, we deduced cell migration pathways in each tumor cell line, and then compared them. As a result, we found that both the MEK/ERK and JNK/c-Jun pathways were activated in all three migrating cell lines. Moreover, GSK-3 and p38 were found to regulate PI3K/Akt pathway in only EC109 cells, and JNK was found to crosstalk with p38 and Fos related pathway in only TT cells. Taken together, our analytical system could easily distinguish between the common and cell type-specific pathways responsible for tumor cell migration.
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DOI：
10.1158/1541-7786.mcr-09-0221
发表时间：
2010-02
期刊：
Molecular cancer research : MCR
影响因子：
--
作者：
Wang J;Kuiatse I;Lee AV;Pan J;Giuliano A;Cui X
通讯作者：
Cui X
影响因子：
37.3
作者：
Makrodouli E;Oikonomou E;Koc M;Andera L;Sasazuki T;Shirasawa S;Pintzas A
通讯作者：
Pintzas A
影响因子：
7.3
作者：
Visser Smit GD;Place TL;Cole SL;Clausen KA;Vemuganti S;Zhang G;Koland JG;Lill NL
通讯作者：
Lill NL
影响因子：
8
作者：
Yue, P.;Zhang, X.;Paladino, D.;Sengupta, B.;Ahmad, S.;Holloway, R. W.;Ingersoll, S. B.;Turkson, J.
通讯作者：
Turkson, J.
影响因子：
4.8
作者：
Pece, S;Chiariello, M;Gutkind, JS
通讯作者：
Gutkind, JS