A faster Rubisco with potential to increase photosynthesis in crops.
A faster Rubisco with potential to increase photosynthesis in crops.
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DOI：
10.1038/nature13776
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发表时间：
2014-09-25
期刊：
影响因子：
64.8
通讯作者：
Hanson, Maureen R.
中科院分区：
文献类型：
--
作者：
Lin, Myat T.;Occhialini, Alessandro;Andralojc, P. John;Parry, Martin A. J.;Hanson, Maureen R.
在光合生物中，d-核酮糖-1，5-二磷酸羧化酶/加氧酶（Rubisco）是将大气CO2同化到生物圈中的主要酶。由于Rubisco的浪费性加氧酶活性和缓慢的周转，该酶是提高维管植物光合效率的最重要靶标之一。将蓝藻的CO2浓缩机制（CCM）引入植物中，可以提高作物产量。然而，Rubisco组装的复杂性质使得该酶的操作极具挑战性，并且试图用来自蓝藻和红藻的酶取代植物中的Rubisco并没有成功。在这里，我们报告了两个转质体烟草株系的功能Rubisco从蓝细菌细长聚球藻PCC 7942（Se 7942）。我们敲除天然烟草基因编码大亚基的Rubisco插入大亚基和小亚基基因的Se 7942酶，与相应的Se 7942组装伴侣，RbcX，或内部carboxysomal蛋白，CcmM 35，其中包括三个小亚基样结构域，。Se 7942 Rubisco和CcmM 35在叶绿体基质中形成大分子复合物，反映了蓝藻β-羧基体生物发生的早期步骤。两个转化株系的光合能力，支持自养生长，和他们各自的形式的Rubisco有较高的CO2固定率每单位的酶比烟草对照。这些转质体烟草系代表了改善植物光合作用的重要一步，并且将是未来添加蓝藻CCM的剩余组分（例如无机碳转运蛋白和β-羧基体壳蛋白）的有价值的宿主。
In photosynthetic organisms,d-ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is the major enzyme assimilating atmospheric CO2into the biosphere. Owing to the wasteful oxygenase activity and slow turnover of Rubisco, the enzyme is among the most important targets for improving the photosynthetic efficiency of vascular plants,. It has been anticipated that introducing the CO2-concentrating mechanism (CCM) from cyanobacteria into plants could enhance crop yield,,. However, the complex nature of Rubisco’s assembly has made manipulation of the enzyme extremely challenging, and attempts to replace it in plants with the enzymes from cyanobacteria and red algae have not been successful,. Here we report two transplastomic tobacco lines with functional Rubisco from the cyanobacteriumSynechococcus elongatusPCC7942 (Se7942). We knocked out the native tobacco gene encoding the large subunit of Rubisco by inserting the large and small subunit genes of the Se7942 enzyme, in combination with either the corresponding Se7942 assembly chaperone, RbcX, or an internal carboxysomal protein, CcmM35, which incorporates three small subunit-like domains,. Se7942 Rubisco and CcmM35 formed macromolecular complexes within the chloroplast stroma, mirroring an early step in the biogenesis of cyanobacterial β-carboxysomes,. Both transformed lines were photosynthetically competent, supporting autotrophic growth, and their respective forms of Rubisco had higher rates of CO2fixation per unit of enzyme than the tobacco control. These transplastomic tobacco lines represent an important step towards improved photosynthesis in plants and will be valuable hosts for future addition of the remaining components of the cyanobacterial CCM, such as inorganic carbon transporters and the β-carboxysome shell proteins,,.
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影响因子：
5.1
作者：
Gray, Benjamin N.;Yang, Huijun;Hanson, Maureen R.
通讯作者：
Hanson, Maureen R.
影响因子：
6.9
作者：
Parry, Martin A. J.;Keys, Alfred J.;Andralojc, P. John
通讯作者：
Andralojc, P. John
影响因子：
7.4
作者：
Whitney, Spencer M.;Houtz, Robert L.;Alonso, Hernan
通讯作者：
Alonso, Hernan
影响因子：
7.4
作者：
McGrath, Justin M.;Long, Stephen P.
通讯作者：
Long, Stephen P.
影响因子：
3.7
作者：
Long, Benedict M.;Rae, Benjamin D.;Price, G. Dean
通讯作者：
Price, G. Dean