Ferrochelatase is a conserved downstream target of the blue light-sensing White collar complex in fungi.
Ferrochelatase is a conserved downstream target of the blue light-sensing White collar complex in fungi.
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DOI：
10.1099/mic.0.039222-0
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发表时间：
2010-08
期刊：
影响因子：
--
通讯作者：
Heitman J
中科院分区：
文献类型：
--
作者：
Idnurm A;Heitman J
光是生物体（包括真菌）感知的普遍信号，其中光根据物种调节常见和独特的生物过程。先前的研究已经证实，来自子囊菌粗糙脉孢菌的保守蛋白（最初被称为白领 1 和 2）可调节紫外线/蓝光传感。同源蛋白在粗糙链孢菌的远亲中发挥作用，包括担子菌和接合菌，它们早在十亿年前就已经分化。在这里，我们对担子菌新型隐球菌进行了微阵列实验，以鉴定光调节基因。令人惊讶的是，只有一个基因被高于常用双阈值的光诱导。该基因 HEM15 预计会编码一种亚铁螯合酶，该酶催化高光反应性卟啉生物合成血红素的最后一步。新型隐球菌基因补充了酿酒酵母 hem15Δ 菌株，对于生存能力至关重要，而 Hem15 蛋白定位于线粒体，这三个证据表明该基因编码亚铁螯合酶。光对 HEM15 的调节表明 bwc1/bwc2 突变体具有光敏感性并表现出毒力降低的机制。我们发现，在粗糙链球菌和接合菌Phycomyces blakesleeanus中，亚铁螯合酶也以白领依赖性方式受到光调节，这表明亚铁螯合酶是真菌界光调节的古老目标。
Light is a universal signal perceived by organisms, including fungi, in which light regulates common and unique biological processes depending on the species. Previous research has established that conserved proteins, originally called White collar 1 and 2 from the ascomycete Neurospora crassa, regulate UV/blue light sensing. Homologous proteins function in distant relatives of N. crassa, including the basidiomycetes and zygomycetes, which diverged as long as a billion years ago. Here we conducted microarray experiments on the basidiomycete fungus Cryptococcus neoformans to identify light-regulated genes. Surprisingly, only a single gene was induced by light above the commonly used twofold threshold. This gene, HEM15, is predicted to encode a ferrochelatase that catalyses the final step in haem biosynthesis from highly photoreactive porphyrins. The C. neoformans gene complements a Saccharomyces cerevisiae hem15Δ strain and is essential for viability, and the Hem15 protein localizes to mitochondria, three lines of evidence that the gene encodes ferrochelatase. Regulation of HEM15 by light suggests a mechanism by which bwc1/bwc2 mutants are photosensitive and exhibit reduced virulence. We show that ferrochelatase is also light-regulated in a white collar-dependent fashion in N. crassa and the zygomycete Phycomyces blakesleeanus, indicating that ferrochelatase is an ancient target of photoregulation in the fungal kingdom.
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影响因子：
11.4
作者：
Chen, Chen-Hui;Ringelberg, Carol S.;Loros, Jennifer J.
通讯作者：
Loros, Jennifer J.
DOI：
10.1016/s0167-4781(96)00227-8
发表时间：
1997-06-26
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
影响因子：
--
作者：
Dutta, S;Gerhold, DL;Kmiec, EB
通讯作者：
Kmiec, EB
影响因子：
3.3
作者：
Harashima, T;Heitman, J
通讯作者：
Heitman, J
DOI：
10.1073/pnas.0600633103
发表时间：
2006-03-21
影响因子：
11.1
作者：
Idnurm, A;Rodríguez-Romero, J;Heitman, J
通讯作者：
Heitman, J
影响因子：
3.1
作者：
Almirón, M;Martínez, M;Ugalde, RA
通讯作者：
Ugalde, RA