Microbial metabolites elicit distinct olfactory and gustatory preferences in bumblebees
Microbial metabolites elicit distinct olfactory and gustatory preferences in bumblebees
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微生物代谢物引起大黄蜂独特的嗅觉和味觉偏好
DOI：
10.1098/rsbl.2019.0132
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发表时间：
2019
期刊：
影响因子：
3.3
通讯作者：
Vannette, Rachel L.
中科院分区：
文献类型：
--
作者：
Schaeffer, Robert N.;Rering, Caitlin C.;Maalouf, Isabelle;Beck, John J.;Vannette, Rachel L.
大黄蜂等动物利用化学感应线索来定位和评估重要资源。人们越来越认识到微生物可以改变觅食资源的质量并产生可作为觅食线索的代谢物。然而，这些化学感应信号的独特性质及其在动物觅食中的用途仍然知之甚少。在这里，我们测试了以下假设：花蜜中的微生物物种通过花蜜中产生的微生物代谢物对授粉昆虫的吸引和取食产生不同的影响。我们首先检查了熊蜂（Bombus impatiens）触角嗅觉神经元对微生物挥发性有机化合物（mVOC）做出反应的电生理潜力，然后进行了嗅觉偏好测试。我们还通过无选择和选择喂养测定来评估对微生物改变的花蜜的味觉偏好。触角嗅觉神经元对一些 mVOC 做出反应，并且蜜蜂更喜欢接种了落新妇细菌的花蜜溶液，而不是仅基于挥发物的酵母 Metschnikowia reukaufii。然而，B.凤仙花采食者消耗了更多的接种 Metschnikowia 的花蜜，这表明 mVOC 和非挥发性代谢物在介导吸引和摄食决策中发挥着独特的作用。总的来说，我们的结果表明，微生物代谢物在塑造种间、植物-传粉媒介信号传导方面具有巨大潜力，对觅食者学习、经济和花卉宿主繁殖产生影响。
Animals such as bumblebees use chemosensory cues to both locate and evaluate essential resources. Increasingly, it is recognized that microbes can alter the quality of foraged resources and produce metabolites that may act as foraging cues. The distinct nature of these chemosensory cues however and their use in animal foraging remain poorly understood. Here, we test the hypothesis that species of nectar-inhabiting microbes differentially influence pollinator attraction and feeding via microbial metabolites produced in nectar. We first examined the electrophysiological potential for bumblebee (Bombus impatiens) antennal olfactory neurons to respond to microbial volatile organic compounds (mVOCs), followed by an olfactory preference test. We also assessed gustatory preferences for microbial-altered nectar through both no-choice and choice feeding assays. Antennal olfactory neurons responded to some mVOCs, and bees preferred nectar solutions inoculated with the bacteriumAsaia astilbesover the yeastMetschnikowia reukaufiibased on volatiles alone. However,B. impatiensforagers consumed significantly moreMetschnikowia-inoculated nectar, suggesting distinct roles for mVOCs and non-volatile metabolites in mediating both attraction and feeding decisions. Collectively, our results suggest that microbial metabolites have significant potential to shape interspecific, plant–pollinator signalling, with consequences for forager learning, economics and floral host reproduction.
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影响因子：
4.8
作者：
Herrera, Carlos M.;Pozo, Maria I.;Medrano, Monica
通讯作者：
Medrano, Monica
飞燕草（毛茛科）花蜜酵母及其对花蜜品质的影响
影响因子：
2.9
作者：
R. Schaeffer;R. Vannette;Rebecca E. Irwin
通讯作者：
Rebecca E. Irwin
影响因子：
9.4
作者：
Rering, Caitlin C.;Beck, John J.;Vannette, Rachel L.
通讯作者：
Vannette, Rachel L.
影响因子：
5.2
作者：
Schaeffer, Robert N.;Mei, Yu Zhu;Irwin, Rebecca E.
通讯作者：
Irwin, Rebecca E.
影响因子：
4.2
作者：
R. Vannette;T. Fukami
通讯作者：
R. Vannette;T. Fukami