BMP-regulated exosomes from Drosophila male reproductive glands reprogram female behavior.
BMP-regulated exosomes from Drosophila male reproductive glands reprogram female behavior.
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DOI：
10.1083/jcb.201401072
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发表时间：
2014-09-01
期刊：
影响因子：
--
通讯作者：
Wilson C
中科院分区：
文献类型：
--
作者：
Corrigan L;Redhai S;Leiblich A;Fan SJ;Perera SM;Patel R;Gandy C;Wainwright SM;Morris JF;Hamdy F;Goberdhan DC;Wilson C
雄性果蝇生殖腺以BMP依赖的方式分泌外泌体，外泌体在交配后与精子融合并抑制雌性再交配。男性生殖腺分泌信号进入精液，以促进生殖成功。在黑腹果蝇中，这些信号是由多种精液肽产生的，其中许多是由附属腺（AG）产生的。成年雄性AG中的一种上皮细胞类型，即次级细胞（SC），响应于骨形态发生蛋白（BMP）信号传导而选择性生长。这种信号参与阻止交配的雌性的快速再交配，这有助于第一个交配的雄性的生殖优势。在本文中，我们表明，SC分泌外泌体，膜结合囊泡内产生的晚期内体多泡体（MVB）。交配后，外泌体与精子融合（如在体外对人前列腺来源的外泌体和精子所见），并与女性生殖道上皮细胞相互作用。需要外泌体释放来抑制雌性再交配行为，这表明外泌体是BMP信号传导的下游效应物。事实上，当BMP信号在SC中减少时，囊泡仍然在MVB中形成，但不作为外泌体分泌。这些结果证明了MVB-外泌体途径在生殖道中的新功能，该功能在进化过程中似乎是保守的。
Male Drosophila reproductive glands secrete exosomes in a BMP-dependent manner that fuse with sperm after mating and suppress female remating. Male reproductive glands secrete signals into seminal fluid to facilitate reproductive success. In Drosophila melanogaster, these signals are generated by a variety of seminal peptides, many produced by the accessory glands (AGs). One epithelial cell type in the adult male AGs, the secondary cell (SC), grows selectively in response to bone morphogenetic protein (BMP) signaling. This signaling is involved in blocking the rapid remating of mated females, which contributes to the reproductive advantage of the first male to mate. In this paper, we show that SCs secrete exosomes, membrane-bound vesicles generated inside late endosomal multivesicular bodies (MVBs). After mating, exosomes fuse with sperm (as also seen in vitro for human prostate-derived exosomes and sperm) and interact with female reproductive tract epithelia. Exosome release was required to inhibit female remating behavior, suggesting that exosomes are downstream effectors of BMP signaling. Indeed, when BMP signaling was reduced in SCs, vesicles were still formed in MVBs but not secreted as exosomes. These results demonstrate a new function for the MVB–exosome pathway in the reproductive tract that appears to be conserved across evolution.
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DOI：
10.1111/tra.12016
发表时间：
2013-01
期刊：
Traffic (Copenhagen, Denmark)
影响因子：
--
作者：
Beckett K;Monier S;Palmer L;Alexandre C;Green H;Bonneil E;Raposo G;Thibault P;Le Borgne R;Vincent JP
通讯作者：
Vincent JP
影响因子：
1.5
作者：
Hayashi, S;Ito, K;Goto, S
通讯作者：
Goto, S
影响因子：
2.7
作者：
DIBENEDETTO, AJ;HARADA, HA;WOLFNER, MF
通讯作者：
WOLFNER, MF
影响因子：
4
作者：
Colombo, Marina;Moita, Catarina;Raposo, Graca
通讯作者：
Raposo, Graca
影响因子：
3.7
作者：
Chanut-Delalande H;Jung AC;Baer MM;Lin L;Payre F;Affolter M
通讯作者：
Affolter M