Label retaining and stem cell marker expression in the developing rat urinary bladder.
Label retaining and stem cell marker expression in the developing rat urinary bladder.
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DOI：
10.1016/j.urology.2011.10.051
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发表时间：
2012-03
期刊：
影响因子：
2.1
通讯作者：
Lin CS
中科院分区：
文献类型：
--
作者：
Zhang H;Lin G;Qiu X;Ning H;Banie L;Lue TF;Lin CS
组织常驻干细胞被认为存在于大多数组织中，它们的鉴定通常是通过对假定的干细胞标记物进行免疫染色和标签保留细胞（LRC）策略的结合来完成的。在本研究中，我们采用这些方法来鉴定膀胱中潜在的干细胞。新生大鼠腹腔注射5-乙基-2-脱氧尿苷（EdU），并在4个不同时间点取膀胱。对膀胱进行EdU染色和干细胞标志物Lgr5、CD34、SSEA-1和c-kit的免疫荧光染色。计数edu阳性细胞，并确定与干细胞标记共定位。edu注射后第1天，每个横截面上标记1804.0±227.7个膀胱细胞。随着时间的增加，被标记的膀胱数量减少，降至236.5±53.0个/场。在1天的膀胱中，27.5±4.9%的上皮细胞被标记，而逼尿肌的这一比例为12.1±2.8%。这两个组织区室的标记率逐渐相等，在8周的样本中达到约5.5%。LRC的分布是随机的，没有优先标记基底细胞。尿路上皮中检测到Lgr5、SSEA-1；CD34和c-kit在固有层和逼尿肌。约30%至40%的c-kit阳性细胞为edu阳性。EdU对膀胱细胞的标记是随机发生的，标记保留与Lgr5、CD34或SSEA-1的表达无关。标签保留和c-kit表达之间的强烈关联似乎与Cajal间质细胞（ICCs）有关，而不是干细胞。
Tissue resident stem cells are believed to exist in most tissues, and their identification is commonly done by a combination of immunostaining for putative stem cell markers and the label-retaining cell (LRC) strategy. In the present study we employed these approaches to identify potential stem cells in the urinary bladder. Newborn rats were intraperitoneally injected with 5-ethynyl-2-deoxyuridine (EdU), and their bladders harvested at four different time points afterward. The bladders were processed for EdU staining and immunofluorescence staining for stem cell markers Lgr5, CD34, SSEA-1, and c-kit. EdU-positive cells were counted and co-localization with stem cell markers determined. At day one post-EdU injection, 1804.0 ± 227.7 bladder cells were labeled in each cross section. As time increased, fewer bladders remained labeled, dropping to 236.5±53.0 cells per field. In the 1-day bladders, 27.5±4.9% of the epithelial cells were labeled as compared to 12.1±2.8% in the detrusor. The labeling rates in these two tissue compartments gradually equalized, reaching at approximately 5.5% in the 8-week samples. Distribution of LRC was random, without preferential labeling of basal cells. Lgr5 and SSEA-1 were detectable in the urothelium; CD34 and c-kit in the lamina propria and detrusor. Approximately 30 to 40% of c-kit-positive cells were EdU-positive. Labeling of bladder cells by EdU occurred randomly, and label retaining was not associated with expression of Lgr5, CD34, or SSEA-1. The strong association between label retaining and c-kit expression appears to relate to interstitial cells of Cajal (ICCs), not stem cells.
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DOI：
10.1016/j.juro.2009.11.012
发表时间：
2010-03
期刊：
The Journal of urology
影响因子：
--
作者：
Huang YC;Shindel AW;Ning H;Lin G;Harraz AM;Wang G;Garcia M;Lue TF;Lin CS
通讯作者：
Lin CS
DOI：
10.1083/jcb.201010131
发表时间：
2011-04-18
期刊：
The Journal of cell biology
影响因子：
--
作者：
Liu L;Rando TA
通讯作者：
Rando TA
影响因子：
4.4
作者：
Thoren, Lina A.;Liuba, Karina;Jacobsen, Sten-Eirik W.
通讯作者：
Jacobsen, Sten-Eirik W.
影响因子：
9.8
作者：
Venezia TA;Merchant AA;Ramos CA;Whitehouse NL;Young AS;Shaw CA;Goodell MA
通讯作者：
Goodell MA
DOI：
10.1152/ajprenal.00533.2007
发表时间：
2008-06-01
影响因子：
4.2
作者：
Kurzrock, Eric A.;Lieu, Deborah K.;Isseroff, Roslyn R.
通讯作者：
Isseroff, Roslyn R.