Effect of Portal Vein Occlusion on the Pancreas: An Experimental Model
Effect of Portal Vein Occlusion on the Pancreas: An Experimental Model
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门静脉阻塞对胰腺的影响：实验模型
DOI：
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发表时间：
2006
影响因子：
2.6
通讯作者：
S. Vatansever
中科院分区：
文献类型：
--
作者：
H. Aydede;Y. Erhan;Ozer Ikgül;S. Cilaker;A. Sakarya;S. Vatansever
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BackgroundThe effects of portal vein occlusion on the pancreas are not clearly understood. Therefore, we studied histomorphological changes induced in the rat pancreas by various periods of portal vein occlusion.Materials and methodsSixty female Wistar albino rats were randomly allocated into four groups of 15 each. In Group I (control), rats underwent sham laparotomy to expose the portal vein proximal to its bifurcation. In Groups II–IV, rats underwent laparotomy followed by portal vein occlusion by clamping for 15, 30, and 60 minutes respectively. The pancreas was removed immediately after sham laparotomy in Group I and immediately after clamp release in Groups II–IV. Pancreatic tissue specimens were subjected to histochemical analysis for cell typing and diagnosis, immunohistochemical analysis for identification of the inflammatory markers tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), endothelial nitric oxide synthase (eNOS), and inducible NOS (iNOS), and TUNEL analysis was carried out for identification of apoptotic cells.ResultsHistochemistry revealed signs of inflammation in pancreatic tissue from rats subjected to portal vein occlusion. Immunohistochemistry revealed that the expression of proinflammatory cytokines TNF-α and IL-1β and the oxidative damage indicator iNOS in rat pancreatic tissue increased progressively with the duration of portal vein occlusion. TUNEL assay revealed no signs of apoptosis in any of the groups.ConclusionWe conclude that portal vein occlusion triggers an inflammatory response in the pancreas that worsens the longer the occlusion lasts.
DOI：
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发表时间：
1988-12
期刊：
The American journal of pathology
影响因子：
--
作者：
J. Pober
通讯作者：
J. Pober
移植后肝网状内皮功能的判别定量。
影响因子：
6.2
作者：
Klein,AS;Zhadkevich,M;Wang,D;Margolick,JB;Winkelstein,JA;Bulkley,GB
通讯作者：
Bulkley,GB
人肝细胞中细胞因子诱导的中性粒细胞趋化因子/IL-8 基因表达。
DOI：
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发表时间：
1990
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Thornton,AJ;Strieter,RM;Lindley,I;Baggiolini,M;Kunkel,SL
通讯作者：
Kunkel,SL