Chromosome 8q24 variants are associated with prostate cancer risk in a high risk population of African ancestry.
Chromosome 8q24 variants are associated with prostate cancer risk in a high risk population of African ancestry.
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DOI：
10.1002/pros.21320
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发表时间：
2011-07
期刊：
影响因子：
2.8
通讯作者：
Bunker, Clareann H.
中科院分区：
文献类型：
--
作者：
Okobia, Michael N.;Zmuda, Joseph M.;Ferrell, Robert E.;Patrick, Alan L.;Bunker, Clareann H.
早期关于种系变异在非洲血统男性前列腺癌负担过高中的作用的研究基本上没有什么回报。然而，最近全基因组关联研究结果的成功复制令人鼓舞，表明染色体 8q24 的某些区域与欧洲和非洲血统男性的不同前列腺癌易感性有关。这项病例对照研究旨在评估非洲裔加勒比多巴哥男性（主要是西非血统的人群）中染色体 8q24 种系变异与前列腺癌风险之间的关联。使用 Qiagen 试剂盒从血凝块中分离出高分子量基因组 DNA。根据制造商的方案，在 7900HT 快速实时 PCR 系统（Applied Biosystems，Foster City，CA）上使用预先设计的 TaqMan SNP 测定法对基因组 DNA 进行基因分型。区域 2 中的 SNP rs16901979 与前列腺癌风险显着增加相关（OR = 1.41，95% 置信区间 [CI] 1.02–1.95，P = 0.04），且早发性前列腺癌男性的风险更高（OR = 2.37，95% CI 1.40–3.99，P = 0.001）。患有早发性前列腺癌的男性中，SNP rs1447295 和 rs6983267 的风险有显着增加的趋势。染色体 8q24 变异与多巴哥男性前列腺癌风险增加之间的关联以及非洲血统人群对照中风险等位基因的较高频率的复制进一步加强了该基因组区域在非洲血统男性前列腺癌负担不成比例的较高中的可能作用。
Earlier studies on the role of germline variations in the disproportionate higher burden of prostate cancer in men of African ancestry have been largely unrewarding. However, the successful replication of recent genome-wide association findings implicating some regions of chromosome 8q24 in the disparate prostate cancer susceptibility in men of European and African ancestry have been encouraging. This case–control study was designed to evaluate the association between germline variations in chromosome 8q24 and prostate cancer risk in Afro-Caribbean Tobago men, a population of predominantly West African ancestry. High molecular weight genomic DNA was isolated from blood clots using Qiagen kits. Genotyping was performed on genomic DNA using a pre-designed TaqMan SNP assay according to the manufacture’s protocol on a 7900HT Fast Real-Time PCR system (Applied Biosystems, Foster City, CA). SNP rs16901979 in region 2 was associated with significantly increased risk of prostate cancer (OR = 1.41, 95% confidence interval [CI] 1.02–1.95, P = 0.04) with the risk stronger in men with early-onset prostate cancer (OR = 2.37, 95% CI 1.40–3.99, P = 0.001). There was a tendency towards significantly increased risk for SNPs rs1447295 and rs6983267 in men with early-onset prostate cancer. The replication of the association of chromosome 8q24 variants with increased prostate cancer risk in Tobago men and the higher frequency of the risk alleles in controls in populations of African ancestry further strengthens the possible role of this genomic region in the disproportionate higher burden of prostate cancer in men of African ancestry.
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DOI：
10.1016/s0197-2456(00)00097-0
发表时间：
2000-12-01
期刊：
CONTROLLED CLINICAL TRIALS
影响因子：
--
作者：
Gohagan, JK;Prorok, PC;Kramer, BS
通讯作者：
Kramer, BS
影响因子：
--
作者：
Ferber, MJ;Eilers, P;Brink, AATP
通讯作者：
Brink, AATP
影响因子：
6.6
作者：
Glover, FE;Coffey, DS;Walsh, PC
通讯作者：
Walsh, PC
影响因子：
1.9
作者：
Valeri, A;Briollais, L;Demenais, F
通讯作者：
Demenais, F
影响因子：
3.7
作者：
van Duin, M;van Marion, R;van Dekken, H
通讯作者：
van Dekken, H