Growth-limiting intracellular metabolites in yeast growing under diverse nutrient limitations.
Growth-limiting intracellular metabolites in yeast growing under diverse nutrient limitations.
复制标题
DOI：
10.1091/mbc.e09-07-0597
复制
发表时间：
2010-01-01
影响因子：
3.3
通讯作者：
Rabinowitz JD
中科院分区：
文献类型：
--
作者：
Boer VM;Crutchfield CA;Bradley PH;Botstein D;Rabinowitz JD
我们发现营养有限的酵母的代谢组随着限制营养的特性而显着变化。低谷氨酰胺是氮限制的标志，ATP 是磷限制的标志，丙酮酸是碳限制的标志。这些代谢物的可用性可以定量地解释营养有限的酵母的生长速度。微生物根据营养物质的可用性调整其生长速度。在这里，我们使用液相色谱-质谱法测量了以五种不同速率和五种不同限制营养素中的每一种生长的酿酒酵母稳态培养物中 > 100 种细胞内代谢物。与基因转录本相反，无论限制性营养素的性质如何，~25% 与生长速率相关，代谢物浓度对限制性营养素的特性高度敏感。氮（铵）和碳（葡萄糖）限制的特点是细胞内氨基酸水平低和核苷酸水平高，而磷（磷酸盐）限制则导致相反的情况。在磷限制中选择性地发现低腺苷酸能量电荷，这表明能量电荷实际上可以衡量磷的可用性。特别强烈的浓度反应发生在与限制性营养物质密切相关的代谢物中，例如氮限制中的谷氨酰胺、磷限制中的ATP和碳限制中的丙酮酸。涉及这些代谢物的可用性的简单但物理上真实的模型足以解释细胞生长速率。完整的数据可以在交互式网站 http://growthrate.princeton.edu/metabolome 上访问。
We find that the metabolome of nutrient-limited yeast varies dramatically with the limiting nutrient's identity. Low glutamine is a hallmark of nitrogen limitation, ATP of phosphorus limitation, and pyruvate of carbon limitation. The availability of these metabolites can quantitatively account for the nutrient-limited yeast's growth rate. Microbes tailor their growth rate to nutrient availability. Here, we measured, using liquid chromatography-mass spectrometry, >100 intracellular metabolites in steady-state cultures of Saccharomyces cerevisiae growing at five different rates and in each of five different limiting nutrients. In contrast to gene transcripts, where ∼25% correlated with growth rate irrespective of the nature of the limiting nutrient, metabolite concentrations were highly sensitive to the limiting nutrient's identity. Nitrogen (ammonium) and carbon (glucose) limitation were characterized by low intracellular amino acid and high nucleotide levels, whereas phosphorus (phosphate) limitation resulted in the converse. Low adenylate energy charge was found selectively in phosphorus limitation, suggesting the energy charge may actually measure phosphorus availability. Particularly strong concentration responses occurred in metabolites closely linked to the limiting nutrient, e.g., glutamine in nitrogen limitation, ATP in phosphorus limitation, and pyruvate in carbon limitation. A simple but physically realistic model involving the availability of these metabolites was adequate to account for cellular growth rate. The complete data can be accessed at the interactive website http://growthrate.princeton.edu/metabolome.
登录
查看更多内容
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
3.6
作者：
Goncalves, PM;Griffioen, G;Planta, RJ
通讯作者：
Planta, RJ
影响因子：
56.9
作者：
HARTWELL, LH;CULOTTI, J;REID, BJ
通讯作者：
REID, BJ
影响因子：
12.3
作者：
Futcher B
通讯作者：
Futcher B
影响因子：
4.8
作者：
Fernández-Murray, JP;McMaster, CR
通讯作者：
McMaster, CR