Effects of extracellular matrix viscoelasticity on cellular behaviour.
Effects of extracellular matrix viscoelasticity on cellular behaviour.
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DOI：
10.1038/s41586-020-2612-2
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发表时间：
2020-08
期刊：
影响因子：
64.8
通讯作者：
Shenoy VB
中科院分区：
文献类型：
--
作者：
Chaudhuri O;Cooper-White J;Janmey PA;Mooney DJ;Shenoy VB
过去二十年的重要研究已经确定，细胞外基质（ECM）弹性或刚度影响基本细胞过程，包括扩散、生长、增殖、迁移、分化和类器官形成。用ECM蛋白包被的线性弹性聚丙烯酰胺水凝胶和聚二甲基硅氧烷（PDMS）弹性体已成为用于评估刚度作用的广泛使用的工具，并且来自这些实验的结果通常被假定为再现细胞在体内经历的机械环境的影响。然而，组织和ECM不是线性弹性材料-它们实际上表现出更复杂的机械行为，包括粘弹性，或对负载或变形的时间依赖性响应，以及机械塑性和非线性弹性。最近的工作表明，基质粘弹性调节这些相同的基本细胞过程，并且重要的是可以促进在2D和3D培养微环境中用弹性水凝胶观察不到的行为。这些重要的发现为细胞-基质相互作用提供了新的见解，并为这些相互作用如何差异调节细胞中的机械敏感分子通路提供了背景。此外，这些结果为下一代生物材料提供了新的设计指南，这些生物材料更好地匹配体外组织模型和再生医学应用中的组织和ECM力学。
Significant research over the past two decades has established that extracellular matrix (ECM) elasticity, or stiffness, impacts fundamental cell processes including spreading, growth, proliferation, migration, differentiation, and organoid formation. Linearly elastic polyacrylamide hydrogels and polydimethylsiloxane (PDMS) elastomers coated with ECM proteins have become widely-used tools for assessing the role of stiffness, and results from these experiments are often assumed to reproduce the effect of the mechanical environment experienced by cells in vivo. However, tissues and ECMs are not linearly elastic materials – they in fact exhibit far more complex mechanical behaviors, including viscoelasticity, or a time-dependent response to loading or deformation, as well as mechanical plasticity and nonlinear elasticity. Recent work has revealed that matrix viscoelasticity regulates these same fundamental cell processes, and importantly can promote behaviors not observed with elastic hydrogels in both 2D and 3D culture microenvironments. These important findings have provided new insights into cell-matrix interactions and have given context as to how these interactions differentially modulate mechano-sensitive molecular pathways in cells. Moreover, these results indicate new design guidelines for the next generation of biomaterials that better match tissue and ECM mechanics for in vitro tissue models and applications in regenerative medicine.
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影响因子：
16.6
作者：
Bangasser BL;Shamsan GA;Chan CE;Opoku KN;Tüzel E;Schlichtmann BW;Kasim JA;Fuller BJ;McCullough BR;Rosenfeld SS;Odde DJ
通讯作者：
Odde DJ
影响因子：
7.6
作者：
Alsberg, E;Kong, HJ;Mooney, DJ
通讯作者：
Mooney, DJ
影响因子：
41.2
作者：
Caiazzo, Massimiliano;Okawa, Yuya;Lutolf, Matthias P.
通讯作者：
Lutolf, Matthias P.
细胞介导的纤维募集驱动工程原纤维微环境中的细胞外基质机械感应。
影响因子：
41.2
作者：
Baker BM;Trappmann B;Wang WY;Sakar MS;Kim IL;Shenoy VB;Burdick JA;Chen CS
通讯作者：
Chen CS
DOI：
10.1073/pnas.0902639106
发表时间：
2009-12-29
影响因子：
11.1
作者：
Bellin, Robert M.;Kubicek, James D.;LeDuc, Philip R.
通讯作者：
LeDuc, Philip R.