Molecular characterization of Camberwell virus and sequence variation in ORF3 of small round‐structured (Norwalk‐like) viruses
Molecular characterization of Camberwell virus and sequence variation in ORF3 of small round‐structured (Norwalk‐like) viruses
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坎伯韦尔病毒的分子特征和小圆形结构（诺沃克样）病毒 ORF3 的序列变异
DOI：
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发表时间：
1996
影响因子：
12.7
通讯作者：
P. J. Wright
中科院分区：
文献类型：
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作者：
M. Cauchi;J. Doultree;J. Marshall;P. J. Wright
对1994年1~11月发生在澳大利亚维多利亚州的5株与胃肠炎有关的小圆结构病毒(SRSV)进行了RT-PCR检测。测定了其中一种病毒Camberwell的基因组3‘半(3.8kb)的序列。Camberwell病毒与布里斯托尔病毒和洛兹代尔病毒亲缘关系最近，属于包含布里斯托尔病毒、洛兹代尔病毒、多伦多病毒、OTH-25病毒、墨西哥病毒和夏威夷病毒的SRSVs基因群。Camberwell和Bristol病毒ORF1(部分)、ORF2和ORF3编码的蛋白质的氨基酸同源性分别为99%、98%和90%。在ORF3中发现了一个高度可变区，对应于123到169个氨基酸残基(布里斯托尔和坎伯韦尔编号)。对其他四种病毒的ORF1(聚合酶区域)短片段和高度可变的ORF3区域进行了分析。所获得的结果表明，可变区在区分密切相关的病毒方面具有潜在的用途。©1996 Wiley-Liss，Inc.
Five small round‐structured viruses (SRSVs) associated with gastroenteritis in Victoria, Australia, from January to November 1994 were examined by sequencing cDNA prepared from faecal samples using RT‐PCR. The sequence of the 3′ half (3.8 kb) of the genome of one of these viruses, Camberwell, was determined. Camberwell virus was related most closely to Bristol and Lordsdale viruses, and belonged to the genetic group of SRSVs containing Bristol, Lordsdale, Toronto, OTH‐25, Mexico, and Hawaii viruses. The amino acid identities between Camberwell and Bristol viruses for proteins encoded by ORF1 (partial), ORF2, and ORF3 were 99%, 98%, and 90%, respectively. A highly variable region in ORF3 corresponding to amino acid residues 123 to 169 (Bristol and Camberwell numbering) were identified. Short segments of ORF1 (polymerase region) and the highly variable ORF3 region was analysed for the other four viruses. The results obtained indicated the potential usefulness of the variable region in distinguishing between closely related viruses. © 1996 Wiley‐Liss, Inc.
影响因子：
3.7
作者：
JIANG, X;WANG, M;ESTES, MK
通讯作者：
ESTES, MK
星状病毒和杯状病毒：新出现的肠道病原体。
DOI：
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发表时间：
1992
期刊：
Infectious agents and disease
影响因子：
--
作者：
Greenberg,HB;Matsui,SM
通讯作者：
Matsui,SM