Signal amplification technology based on entropy-driven molecular switch for ultrasensitive electrochemical determination of DNA and Salmonella typhimurium
Signal amplification technology based on entropy-driven molecular switch for ultrasensitive electrochemical determination of DNA and Salmonella typhimurium
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基于熵驱动分子开关的信号放大技术用于超灵敏电化学测定DNA和鼠伤寒沙门氏菌
DOI：
10.1016/j.snb.2015.11.086
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发表时间：
2016-03
期刊：
影响因子：
--
通讯作者：
Hun Xu
中科院分区：
文献类型：
--
作者：
Zong Yingxia;Liu Fang;Zhang Yue;Zhan Tianrong;He Yunhua;Hun Xu
一种基于熵驱动分子开关信号放大策略的方法已被开发用于 DNA 的超灵敏检测。用金纳米颗粒修饰的金电极用于固定捕获发夹 DNA。在目标 DNA 存在的情况下，一旦捕获 DNA 和目标 DNA 之间发生杂交反应，发夹 DNA 的茎就会打开。通过添加与捕获DNA有更多互补碱基的链接DNA，链接DNA将与捕获DNA杂交，并且目标DNA将在熵驱动下发生位移。释放的目标DNA可以打开另一个发夹DNA。通过这样的循环，目标DNA可以被回收，更多的发夹DNA被打开，更多的链接DNA将与捕获的DNA杂交。当加入由纳米颗粒、探针DNA和电化学试剂组成的电化学纳米颗粒探针时，连接DNA将与探针DNA杂交。因此，可以放大和监测电化学响应。在这项工作中，选择鼠伤寒沙门氏菌适体互补DNA作为模型靶标，并且S.还通过靶标诱导链释放技术结合熵驱动分子开关信号放大策略对鼠伤寒杆菌进行了测试。该电化学传感器表现出优异的传感性能，如超低检测限（DNA为0.3 fmol L−1，鼠伤寒沙门氏菌为13 cfu mL−1）和高特异性，表明它非常有希望为DNA和S.提供一种灵敏、选择性、成本效益高且方便的方法。鼠伤寒杆菌检测。
A methodology based on entropy-driven molecule switch signal amplification strategy has been developed for the ultrasensitive detection of DNA. The gold electrode modified with gold nanoparticles was used to immobilize capture hairpin DNA. In the presence of target DNA, the stem of hairpin DNA was opened once the hybridization reaction occurred between the capture DNA and target DNA. With the addition of the link DNA which has more complementary bases with the capture DNA, the link DNA would hybridize with the capture DNA, and the target DNA would be displaced under the entropy-driven. The released target DNA could open another hairpin DNA. Through such a cycle the target DNA could be recycled, and more hairpin DNA was opened and more link DNA would hybridize with the capture DNA. When the electrochemical nanoparticle probe which consisted of the nanoparticles, probe DNA and the electrochemical reagent was added, the link DNA would hybridize with the probe DNA. As a result, the electrochemical response can be amplified and monitored. In this work, theSalmonella typhimuriumaptamer complementary DNA was chosen as a model target, andS. typhimuriumwas also tested by target induced strand release technology coupling the entropy-driven molecule switch signal amplified strategy. The electrochemical sensor demonstrated excellent sensing performances such as ultra low detection limit (0.3 fmol L−1for DNA and 13 cfu mL−1forS. typhimurium) and high specificity, indicating that it is highly promising to provide a sensitive, selective, cost-effective, and convenient approach for DNA andS. typhimuriumdetection.
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影响因子：
6.1
作者：
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影响因子：
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