The SAC3 gene encodes a nuclear protein required for normal progression of mitosis.
The SAC3 gene encodes a nuclear protein required for normal progression of mitosis.
复制标题
SAC3 基因编码有丝分裂正常进行所需的核蛋白。
作者：
A. Bauer;R. Kölling
酿酒酵母的SAC 3基因与肌动蛋白功能有关，遗传实验表明，必需肌动蛋白基因（actl-1）中的温度敏感性突变可以被SAC 3中的突变抑制。SAC 3突变体中肌动蛋白细胞骨架改变的观察结果进一步表明SAC 3参与肌动蛋白功能。然而，我们的分馏实验指向Sac 3 p的核定位。在蔗糖密度梯度上，Sac 3 p与所检查的核细胞器标记物共分级。此外，Sac 3 p与核蛋白Nop 1 p沿着在核制备物中富集10倍。在这份报告中，我们进一步表明，SAC 3的功能是需要正常的有丝分裂的进展。SAC 3突变体在培养中显示出更高比例的大出芽细胞，表明细胞周期延迟。在大出芽的sac 3细胞中，主要群体是在芽颈处具有单核和短核内纺锤体的细胞。这表明细胞周期延迟发生在有丝分裂后期之前。SAC 3突变体以比野生型更高的频率丢失染色体的观察结果是SAC 3突变体中有丝分裂缺陷的另一个指示。我们进一步注意到，SAC 3突变体对微管不稳定药物苯菌灵更具抗性。这一发现表明SAC 3直接或间接参与微管功能。总之，我们的数据表明，SAC 3参与了一个影响肌动蛋白细胞骨架和有丝分裂的过程。
The SAC3 gene of Saccharomyces serevisiae has been implicated in actin function by genetic experiments showing that a temperature sensitive mutation in the essential actin gene (actl-1) can be suppressed by mutations in SAC3. An involvement of SAC3 in actin function is further suggested by the observation that the actin cytoskeleton is altered in SAC3 mutants. Our fractionation experiments, however, point to a nuclear localization of Sac3p. On sucrose density gradients Sac3p co-fractionated with the nuclear organelle markers examined. Furthermore, Sac3p was enriched 10-fold in a nuclei preparation along with the nuclear protein Nop1p. In this report we further show that SAC3 function is required for normal progression of mitosis. SAC3 mutants showed a higher fraction of large-budded cells in culture, indicative of a cell cycle delay. The predominant population among the large-budded sac3 cells were cells with a single nucleus at the bud-neck and a short intranuclear spindle. This suggests that a cell cycle delay occurs in mitosis prior to anaphase. The observation that SAC3 mutants lose chromosomes with higher frequency than wild-type is another indication for a mitotic defect in SAC3 mutants. We further noticed that SAC3 mutants are more resistant against the microtubule destabilizing drug benomyl. This finding suggests that SAC3 is involved, directly or indirectly, in microtubule function. In summary, our data indicate that SAC3 is involved in a process which affects both the actin cytoskeleton and mitosis.
登录
查看更多内容
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Dieckmann,CL;Tzagoloff,A
通讯作者：
Tzagoloff,A
影响因子：
--
作者：
J. Pringle;R. Preston;A. Adams;T. Stearns;D. Drubin;B. Haarer;E. Jones
通讯作者：
J. Pringle;R. Preston;A. Adams;T. Stearns;D. Drubin;B. Haarer;E. Jones
影响因子：
3.3
作者：
P. Novick;B. Osmond;D. Botstein
通讯作者：
P. Novick;B. Osmond;D. Botstein
影响因子：
3.3
作者：
L. Hartwell;David M. Smith
通讯作者：
L. Hartwell;David M. Smith
酵母 F1-ATPase β 亚基前体含有功能冗余的线粒体蛋白输入信息。
影响因子：
5.3
作者：
Bedwell,DM;Klionsky,DJ;Emr,SD
通讯作者：
Emr,SD