Identification and Characterization of Novel circRNAs Involved in Muscle Growth of Blunt Snout Bream (Megalobrama amblycephala).
Identification and Characterization of Novel circRNAs Involved in Muscle Growth of Blunt Snout Bream (Megalobrama amblycephala).
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DOI：
10.3390/ijms221810056
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发表时间：
2021-09-17
影响因子：
5.6
通讯作者：
Gao Z
中科院分区：
文献类型：
--
作者：
Liu L;Chen Y;Diao J;Luo L;Gao Z
环状RNA（circular RNA，circRNA）是一类新型的内源性RNA，在动物的生长发育过程中起着重要的作用。然而，circRNA对鱼类肌肉生长的调控机制尚不清楚。本研究以团头鲂（Megalobrama amblycephala）为研究对象，对两个生长速度不同的种群（快生长和慢生长）的骨骼肌进行了全基因组分析。通过qPCR和桑格测序验证选择的circRNA。利用预测的差异表达（DE）对构建circRNA-miRNA-mRNA网络，揭示其在肌肉成肌和肥大中的调控作用。结果，共鉴定出445个circRNA，包括快速生长（FG）和缓慢生长（SG）组之间的42个DE circRNA。这些DE circRNA中的许多与氨基聚糖的生物合成和代谢过程、细胞动力学过程以及粘附连接途径相关。功能预测结果显示，novel_circ_0001608和novel_circ_0002886与dre-miR-153 b-5 p和dre-miR-124-6- 5 p竞争结合，可能作为竞争性内源RNA（ceRNA）分别调控MamblycephalaGene 14755（pik 3r 1）和MamblycephalaGene 10444（apip）水平，从而在肌肉生长中发挥重要调控作用。这些结果不仅有助于我们进一步了解M.同时也为研究circRNA调控鱼类生长和肌肉发育的潜在机制提供了新的线索。
Circular RNAs (circRNAs), a novel class of endogenous RNAs, have been recognized to play important roles in the growth of animals. However, the regulatory mechanism of circRNAs on fish muscle growth is still unclear. In this study, we performed whole transcriptome analysis of skeletal muscles from two populations with different growth rates (fast-growing and slow-growing) of blunt snout bream (Megalobrama amblycephala), an important fish species for aquaculture. The selected circRNAs were validated by qPCR and Sanger sequencing. Pairs of circRNA–miRNA–mRNA networks were constructed with the predicted differentially expressed (DE) pairs, which revealed regulatory roles in muscle myogenesis and hypertrophy. As a result, a total of 445 circRNAs were identified, including 42 DE circRNAs between fast-growing (FG) and slow-growing (SG) groups. Many of these DE circRNAs were related with aminoglycan biosynthetic and metabolic processes, cytokinetic processes, and the adherens junction pathway. The functional prediction results showed that novel_circ_0001608 and novel_circ_0002886, competing to bind with dre-miR-153b-5p and dre-miR-124-6-5p, might act as competing endogenous RNAs (ceRNAs) to control MamblycephalaGene14755 (pik3r1) and MamblycephalaGene10444 (apip) level, respectively, thus playing an important regulatory role in muscle growth. Overall, these results will not only help us to further understand the novel RNA transcripts in M. amblycephala, but also provide new clues to investigate the potential mechanism of circRNAs regulating fish growth and muscle development.
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