Dose-dependent effect of isoflurane on neurovascular coupling in rat cerebral cortex.
Dose-dependent effect of isoflurane on neurovascular coupling in rat cerebral cortex.
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DOI：
10.1111/j.1460-9568.2009.06812.x
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发表时间：
2009-07
期刊：
影响因子：
--
通讯作者：
Kim SG
中科院分区：
文献类型：
--
作者：
Masamoto K;Fukuda M;Vazquez A;Kim SG
神经血管耦合的研究被广泛进行麻醉动物使用功能性磁共振成像（fMRI）。在这项研究中，异氟醚对神经血管耦合的剂量依赖性影响进行了研究，同时记录的局部场电位（FP）和脑血流量（CBF）在大鼠体感皮层。使用电前爪刺激，并且由不同频率的单个脉冲或十个脉冲组成。我们观察到，FP对单脉冲刺激的反应在测试的不同异氟烷水平下（1.1%至2.1%）不受影响，而CBF对单脉冲刺激的反应呈剂量依赖性增加（7 ± 3%至17 ± 4%）。异氟烷剂量不影响高碳酸血症激发诱导的血管反应性。这些结果表明，异氟烷的作用部位影响神经血管机制。对于十脉冲刺激，FP反应总和随着异氟烷剂量的增加而单调下降，可能是由于FP适应的增强。与此相反，剂量依赖性效应的CBF反应不同，这取决于刺激频率; CBF反应的剂量依赖性降低，观察到高频刺激，而剂量依赖性增加，观察到低频刺激。此外，由单脉冲血流动力学脉冲响应与十脉冲FP记录卷积组成的线性时不变模型表明，神经血管传递函数因高频刺激的异氟烷剂量而改变。这些结果表明，当比较从不同动物或生理和药理学操作获得的fMRI数据时，需要仔细和一致地维持麻醉深度。
Neurovascular coupling studies are widely conducted in anesthetized animals using functional magnetic resonance imaging (fMRI). In this study, the dose-dependent effects of isoflurane on the neurovascular coupling were examined with concurrent recordings of the local field potential (FP) and cerebral blood flow (CBF) in the rat somatosensory cortex. Electrical forepaw stimulation was used and consisted of either a single pulse or ten pulses at various frequencies. We observed that the FP response to single-pulse stimulation remained unaffected across the different levels of isoflurane tested (1.1% to 2.1%), while the CBF response to single-pulse stimulation increased dose-dependently (7 ± 3% to 17 ± 4%). The isoflurane dose did not affect the vascular reactivity induced by a hypercapnic challenge. These findings suggest that the action site of isoflurane affects the neurovascular mechanisms. For ten-pulse stimulation, the sum-FP responses monotonically decreased with an increase in the isoflurane dose, possibly due to an enhancement in the FP adaptation. In contrast, the dose-dependent effect on the CBF response varied depending on the stimulus frequency; a dose-dependent decrease in the CBF response was observed for high-frequency stimulation, whereas a dose-dependent increase was observed for low-frequency stimulation. Further, a linear time-invariant model composed of the single-pulse hemodynamic impulse response convoluted with ten-pulse FP recordings showed that the neurovascular transfer function was altered by the isoflurane dose for high-frequency stimulation. These results indicate that a careful and consistent maintenance of the anesthetic depth is required when comparing fMRI data obtained from different animals or physiological and pharmacological manipulations.
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