Transcriptional responses underlying the hormetic and detrimental effects of the plant secondary metabolite gossypol on the generalist herbivore Helicoverpa armigera.
Transcriptional responses underlying the hormetic and detrimental effects of the plant secondary metabolite gossypol on the generalist herbivore Helicoverpa armigera.
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DOI：
10.1186/1471-2164-12-575
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发表时间：
2011-11-23
期刊：
影响因子：
4.4
通讯作者：
Heckel DG
中科院分区：
文献类型：
--
作者：
Celorio-Mancera Mde L;Ahn SJ;Vogel H;Heckel DG
兴奋作用是一种双相生物反应，其特征是在相对较低的浓度下产生刺激作用，而高浓度的化合物则是有害的。棉酚是一种有毒的代谢物，存在于锦葵科一些植物的色素腺体中。棉酚是一种有毒的代谢物，在取食棉花的昆虫中观察到幼虫生长速度的节律反应。通过在棉铃虫饲料中加入不同剂量的棉酚，研究了棉酚对棉铃虫(Helicoverpa Armigera)的发育影响。此外，我们试图确定不同剂量棉酚的潜在转录反应。在饲养试验中，对幼虫增重、蛹重和幼虫发育时间进行了测定，前两个性状表现为节律反应。根据幼虫对棉酚的净增重反应，选择0.016%、0.16%和0.016%三种浓度的棉酚在肠道和身体其他部位进行基因转录谱双色双参考芯片实验。在转录水平上可以观察到兴奋作用，因为在低棉酚剂量下，参与能量获取的基因，如β-呋喃果糖苷酶在肠道中上调，而参与细胞黏附的基因在体内下调。体内有害剂量的治疗显著影响了那些被预测为与膜整合或与蛋白酶体核心复合体相关的产物的基因。观察到在最高剂量的棉酚作用下，氧化还原酶活性相关基因在两种组织中都发生了显著的变化。这项研究代表了第一种转录图谱方法，研究了不同浓度的棉酚对鳞翅目物种的影响。棉铃虫对棉酚摄食的转录反应是组织和剂量依赖的，涉及不同的解毒机制，不仅可以减轻棉酚的直接影响，还可以减轻间接损伤，如pH紊乱和氧自由基的形成。通过这种转录方法发现的基因可能是了解棉酚解毒和应对棉酚诱导的压力的额外候选基因。在一种已经进化出对各种不同植物化合物的转录调节反应的多面性食草动物中，兴奋作用可能是由于较低的促进生长、应对压力反应的诱导阈值和较高的解毒途径的诱导阈值，这些途径代价高昂，并对细胞造成附带损害。
Hormesis is a biphasic biological response characterized by the stimulatory effect at relatively low amounts of chemical compounds which are otherwise detrimental at higher concentrations. A hormetic response in larval growth rates has been observed in cotton-feeding insects in response to increasing concentrations of gossypol, a toxic metabolite found in the pigment glands of some plants in the family Malvaceae. We investigated the developmental effect of gossypol in the cotton bollworm, Helicoverpa armigera, an important heliothine pest species, by exposing larvae to different doses of this metabolite in their diet. In addition, we sought to determine the underlying transcriptional responses to different gossypol doses. Larval weight gain, pupal weight and larval development time were measured in feeding experiments and a hormetic response was seen for the first two characters. On the basis of net larval weight gain responses to gossypol, three concentrations (0%, 0.016% and 0.16%) were selected for transcript profiling in the gut and the rest of the body in a two-color double reference design microarray experiment. Hormesis could be observed at the transcript level, since at the low gossypol dose, genes involved in energy acquisition such as β-fructofuranosidases were up-regulated in the gut, and genes involved in cell adhesion were down-regulated in the body. Genes with products predicted to be integral to the membrane or associated with the proteasome core complex were significantly affected by the detrimental dose treatment in the body. Oxidoreductase activity-related genes were observed to be significantly altered in both tissues at the highest gossypol dose. This study represents the first transcriptional profiling approach investigating the effects of different concentrations of gossypol in a lepidopteran species. H. armigera's transcriptional response to gossypol feeding is tissue- and dose-dependent and involves diverse detoxifying mechanisms not only to alleviate direct effects of gossypol but also indirect damage such as pH disturbance and oxygen radical formation. Genes discovered through this transcriptional approach may be additional candidates for understanding gossypol detoxification and coping with gossypol-induced stress. In a generalist herbivore that has evolved transcriptionally-regulated responses to a variety of different plant compounds, hormesis may be due to a lower induction threshold of growth-promoting, stress-coping responses and a higher induction threshold of detoxification pathways that are costly and cause collateral damage to the cell.
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