Ethyl-substitutive Thioflavin T as a highly-specific fluorescence probe for detecting G-quadruplex structure.
Ethyl-substitutive Thioflavin T as a highly-specific fluorescence probe for detecting G-quadruplex structure.
复制标题
乙基取代硫磺素 T 作为高特异性荧光探针用于检测 G-四链体结构
DOI：
10.1038/s41598-018-20960-7
复制
发表时间：
2018-02-08
影响因子：
4.6
通讯作者：
Tang YL
中科院分区：
文献类型：
--
作者：
Guan AJ;Zhang XF;Sun X;Li Q;Xiang JF;Wang LX;Lan L;Yang FM;Xu SJ;Guo XM;Tang YL
由于G-四链体广泛存在于基因组功能区和转录本中，对端粒维持、基因转录和基因翻译等生物学过程有重要影响，因此引起了人们的广泛关注。人工受体的研究对G-四链体相关的基础研究、临床诊断和治疗具有重要意义，因此，人工受体的研究是从DNA物种中准确鉴定G-四链体的重要手段。在此，荧光染料ThT-E，已知的荧光探针硫磺素T（ThT）的衍生物，被设计和合成，以有效地区分各种G-四链体结构从其他核酸形式。与ThT中的甲基相比，ThT-E中引入了三个乙基，这导致了更强的亲和力和选择性，并且对G-四链体的形成几乎没有诱导作用。更重要的是，ThT-E可以作为一个视觉工具，直接区分G-四链体溶液，甚至在紫外光照射下用肉眼。因此，本文报道的探针可能具有很大的希望，用于高通量检测以筛选G-四链体，这可能广泛地应用于基于G-四链体的潜在诊断和治疗。
G-quadruplex has attracted considerable attention due to their prevalent distribution in functional genomic regions and transcripts, which can importantly influence biological processes such as regulation of telomere maintenance, gene transcription and gene translation. Artificial receptor study has been developed for accurate identification of G-quadruplex from DNA species, since it is important for the G-quadruplex related basic research, clinical diagnosis, and therapy. Herein, fluorescent dye ThT-E, a derivative of the known fluorescence probe Thioflavin T (ThT), was designed and synthesized to effectively differentiate various G-quadruplex structures from other nucleic acid forms. Compared with methyl groups in ThT, three ethyl groups were introduced to ThT-E, which leads to strengthened affinity, selectivity and little inducing effect on the G-quadruplex formation. More importantly, ThT-E could be served as a visual tool to directly differentiate G-quadruplex solution even with naked eyes under illumination of ultraviolet light. Thus, this probe reported herein may hold great promise for high-throughput assay to screen G-quadruplex, which may widely apply to G-quadruplex-based potential diagnosis and therapy.
登录
查看更多内容
影响因子：
14.9
作者：
Renaud de la Faverie A;Guédin A;Bedrat A;Yatsunyk LA;Mergny JL
通讯作者：
Mergny JL
影响因子：
4.8
作者：
Lammich, Sven;Kamp, Frits;Haass, Christian
通讯作者：
Haass, Christian
影响因子：
16.6
作者：
Bhasikuttan, Achikanath C.;Mohanty, Jyotirmayee;Pal, Haridas
通讯作者：
Pal, Haridas
影响因子：
15
作者：
Dexheimer, TS;Sun, D;Hurley, LH
通讯作者：
Hurley, LH
影响因子：
64.5
作者：
HENDERSON, E;HARDIN, CC;BLACKBURN, EH
通讯作者：
BLACKBURN, EH