Expression and role of GPR87 in urothelial carcinoma of the bladder.
Expression and role of GPR87 in urothelial carcinoma of the bladder.
复制标题
GPR87在膀胱的尿路上皮癌中的表达和作用。
DOI：
10.3390/ijms140612367
复制
发表时间：
2013-06-10
影响因子：
5.6
通讯作者：
Kakehi Y
中科院分区：
文献类型：
--
作者：
Okazoe H;Zhang X;Liu D;Shibuya S;Ueda N;Sugimoto M;Kakehi Y
孤儿 GPR87 最近与其配体 LPA 相匹配，LPA 是一种具有多种生理功能（包括癌细胞增殖）的脂质介质。本研究旨在阐明GPR87在膀胱尿路上皮癌中的作用。在七种人膀胱癌细胞系中评估了 GPR87 的表达。构建了表达靶向 GPR87 的 shRNA (Ad-shGPR87) 的复制缺陷型重组腺病毒载体。使用Ad-shGPR87进行基因沉默。对 71 名非肌层浸润性膀胱癌患者的经尿道切除膀胱肿瘤样本进行了免疫组织化学分析。我们在七个细胞系中的五个中观察到 GPR87 表达，沉默 GPR87 基因表达会显着降低细胞活力。 71 个肿瘤中有 38 个（54%）GPR87 表达呈阳性。 Ki-67 指数与 GPR87 染色阳性状态相关 (p < 0.0001)。 GPR87 阳性肿瘤患者的膀胱内无复发生存期比 GPR87 阴性肿瘤患者短 (p = 0.010)。多变量分析显示，GPR87 染色状态是膀胱内复发的独立预后参数 (p = 0.041)。在 GPR87 阳性肿瘤患者中更频繁地观察到从非肌肉侵袭性肿瘤进展到肌肉侵袭性肿瘤，尽管这种趋势并未达到统计学显着性 (p = 0.056)。这些结果需要进一步的前瞻性研究来阐明 GPR87 表达在膀胱癌膀胱内复发和进展中的作用。
The orphan GPR87 has recently been matched with its ligand LPA, which is a lipid mediator with multiple physiological functions, including cancer cell proliferation. This study aimed to clarify the role of GPR87 in urothelial carcinoma of the bladder. GPR87 expression was assessed in seven human bladder cancer cell lines. A replication-deficient recombinant adenoviral vector expressing shRNA targeting GPR87 (Ad-shGPR87), was constructed. Gene silencing was carried out using Ad-shGPR87. Immunohistochemical analysis was performed for transurethral resection of bladder tumor samples from 71 patients with non-muscle-invasive bladder cancer. We observed GPR87 expression in five of the seven cell lines, and silencing GPR87 gene expression significantly reduced cell viability. GPR87 expression was positive in 38 (54%) of 71 tumors. Ki-67 index was associated with positive GPR87 staining status (p < 0.0001). Patients with GPR87-positive tumors had shorter intravesical recurrence-free survival than those with GPR87-negative tumors (p = 0.010). Multivariate analysis revealed that GPR87 staining status was an independent prognostic parameter for intravesical recurrence (p = 0.041). Progression from non-muscle-invasive to muscle-invasive tumor was more frequently observed in patients with GPR87-positive tumors, although this trend did not reach statistical significance (p = 0.056). These results warrant further prospective studies to clarify the role of GPR87 expression in intravesical recurrence and progression in bladder cancer.
登录
查看更多内容
影响因子：
11.2
作者：
Ward Y;Lake R;Yin JJ;Heger CD;Raffeld M;Goldsmith PK;Merino M;Kelly K
通讯作者：
Kelly K
影响因子：
--
作者：
Gugger M;White R;Song S;Waser B;Cescato R;Rivière P;Reubi JC
通讯作者：
Reubi JC
影响因子：
6.4
作者：
Santos, L;Amaro, T;Lopes, C
通讯作者：
Lopes, C
DOI：
10.1016/j.bbrc.2007.09.063
发表时间：
2007-11-23
影响因子：
3.1
作者：
Tabata, Ken-Ichi;Baba, Kiyoshi;Fujita, Norihisa
通讯作者：
Fujita, Norihisa
影响因子：
2.8
作者：
Zeng, Yu;Kakehi, Yoshiyuki;Wu, Xiu-Xian
通讯作者：
Wu, Xiu-Xian