GPR87 is an overexpressed G-protein coupled receptor in squamous cell carcinoma of the lung.
GPR87 is an overexpressed G-protein coupled receptor in squamous cell carcinoma of the lung.
复制标题
DOI：
10.1155/2008/857474
复制
发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Reubi JC
中科院分区：
文献类型：
--
作者：
Gugger M;White R;Song S;Waser B;Cescato R;Rivière P;Reubi JC
肺癌是全球癌症死亡的主要原因。治疗后的5年总生存率约为16%，显然需要更好的治疗选择，例如靶向特定分子结构的治疗。G蛋白偶联受体（GPCR）是细胞表面受体中最大的家族，是一组重要的潜在的诊断和治疗靶点。因此，我们使用激光捕获显微切割和GPCR聚焦的Affyssin微阵列来检测10例鳞状细胞癌和7例腺癌患者组织样本中929个GPCR转录本的表达，以确定非小细胞肺癌（NSCLC）的新靶点。计算每个患者肿瘤样本与相邻肺泡组织样本的相对基因表达水平。基于这种独特的研究设计，我们在鳞状细胞癌中鉴定了5种显著过表达的GPCR，表达的降序为：GPR 87> CMKOR 1> FZD 10> LGR 4> P2 RY 11。所有这些都是非嗅觉和GRAFS（谷氨酸，视紫红质，粘附，卷曲/味道2，分泌素家族）分类。GPR 87、LGR 4和CMKOR 1是孤儿受体。GPR 87由于其显著的过表达和在基于突变的水平上与鳞状细胞癌的相关性而脱颖而出作为进一步靶点验证的候选者。
Lung cancer is the leading cause of cancer death worldwide. The overall 5-year survival after therapy is about 16% and there is a clear need for better treatment options, such as therapies targeting specific molecular structures. G-protein coupled receptors (GPCRs), as the largest family of cell surface receptors, represent an important group of potential targets for diagnostics and therapy. We therefore used laser capture microdissection and GPCR-focused Affymetrix microarrays to examine the expression of 929 GPCR transcripts in tissue samples of 10 patients with squamous cell carcinoma and 7 with adenocarcinoma in order to identify novel targets in non-small cell lung carcinoma (NSCLC). The relative gene expression levels were calculated in tumour samples compared to samples of the neighbouring alveolar tissue in every patient. Based on this unique study design, we identified 5 significantly overexpressed GPCRs in squamous cell carcinoma, in the following decreasing order of expression: GPR87 > CMKOR1 > FZD10 > LGR4 > P2RY11. All are non-olfactory and GRAFS (glutamate, rhodopsin, adhesion, frizzled/taste2, secretin family) classified. GPR87, LGR4 and CMKOR1 are orphan receptors. GPR87 stands out as a candidate for further target validation due to its marked overexpression and correlation on a mutation-based level to squamous cell carcinoma.
登录
查看更多内容
影响因子：
8
作者：
Kerley-Hamilton, JS;Pike, AM;Spinella, MJ
通讯作者：
Spinella, MJ
影响因子：
254.7
作者：
Parkin, DM;Bray, F;Pisani, P
通讯作者：
Pisani, P
影响因子：
--
作者：
Kettunen, E;El-Rifai, W;Knuutila, S
通讯作者：
Knuutila, S
影响因子：
3.7
作者：
Pei, JM;Balsara, BR;Testa, JR
通讯作者：
Testa, JR
影响因子：
--
作者：
Mazerbourg, S;Bouley, DM;Hsueh, AJW
通讯作者：
Hsueh, AJW